Laboratory of Fisheries and Aquaculture, Department of Zoology, Kohat University of Science and Technology, Kohat, Pakistan.
Institute of System Biology (INBIOSIS), Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor, Malaysia.
J Fish Dis. 2023 Nov;46(11):1225-1237. doi: 10.1111/jfd.13841. Epub 2023 Jul 27.
This study explored the bactericidal role of the epidermal mucus (EM) of five freshwater Cyprinid fish species namely Ctenopharyngodon idella, Labeo rohita, Catla catla, Hypophthalmichthys molitrix, and Cirrhinus mrigala after treatment with Aeromonas hydrophila. Extracts of EM (crude and acidic) of each species showed bactericidal activity against various Gram -ve (Pseudomonas aeruginosa, Escherichia coli, Aeromonas hydrophila, Edwardsiella tarda, Salmonella enterica, Klebsiella pneumonia, Serratia marcescens, and Enterobacter cloacae) and Gram +ve (Bacillus wiedmannii and Staphylococcus aureus) bacteria compared with standard antibiotics (Fosfomycin). The zone of inhibition (ZOI) was measured in millimetres against antibiotics (Fosfomycin). Variations in bactericidal activity of EM were observed against bacteria from the same and different fish species. The acidic extract was more effective than the crude extract and showed significantly higher ZOI values against various bacteria and Fosfomycin antibiotics. This result shows that fish EM may perform an important role in fish defence against bacteria. Therefore, this study may hint towards the substitution of synthetic antibiotics with fish EM that may be used as a novel 'bactericidal' in aquaculture as well as in humans against bacterial infections.
本研究探讨了五种淡水鲤科鱼类（草鱼、鲤鱼、鲤鱼、鲤鱼和鲤鱼）表皮黏液（EM）在经过嗜水气单胞菌处理后的杀菌作用。 每个物种的 EM（粗提物和酸性提取物）对各种革兰氏阴性（铜绿假单胞菌、大肠杆菌、嗜水气单胞菌、爱德华氏菌、肠炎沙门氏菌、肺炎克雷伯菌、粘质沙雷氏菌和阴沟肠杆菌）和革兰氏阳性（魏氏芽孢杆菌和金黄色葡萄球菌）细菌均显示出杀菌活性，与标准抗生素（磷霉素）相比。 抑菌圈（ZOI）以毫米为单位针对抗生素（磷霉素）进行测量。 观察到 EM 对来自相同和不同鱼类物种的细菌的杀菌活性存在差异。 酸性提取物比粗提物更有效，对各种细菌和磷霉素抗生素表现出更高的 ZOI 值。 该结果表明，鱼类 EM 可能在鱼类抵抗细菌方面发挥重要作用。 因此，本研究可能暗示可以用鱼类 EM 替代合成抗生素，将其作为一种新型的“杀菌”物质，用于水产养殖以及人类对抗细菌感染。
