Structure-function relationships of phospholipases. I: Prediction of presynaptic neurotoxicity.

The hydropathic indexes of 24 phospholipases have been calculated from their amino acid sequences. The presynaptic neurotoxic potential of venom phospholipases can be predicted by the use of hydropathy profiles. The presynaptically acting phospholipases have a distinct hydrophobic region around the residues 80-110 which is probably involved in the interaction with the presynaptic membranes. This region is present as a separate helix in the tertiary structure of phospholipases. Such a hydrophobic region is absent in non-neurotoxic phospholipases.