Frangioni G, Borgioli G
Stain Technol. 1986 Jul;61(4):239-42. doi: 10.3109/10520298609109944.
Complete bleaching of melanin in strongly pigmented specimens embedded in paraffin or polystyrene, and sectioned and mounted on slides, is possible in 1-3 hr at 37 C in a solution of 20 ml of benzyl alcohol, 10 ml of acetone, 5 ml of 10% hydrogen peroxide and 4 drops of a 25% ammonia solution. The bleached tissues are well preserved and tolerate further histochemical treatments. All the stains and reactions tested give results identical to or better than those obtained after 24-48 hr oxidation in 10% hydrogen peroxide.
将包埋在石蜡或聚苯乙烯中、切片并安装在载玻片上的色素沉着强烈的标本中的黑色素完全漂白，在37℃下，于由20毫升苯甲醇、10毫升丙酮、5毫升10%过氧化氢和4滴25%氨溶液组成的溶液中，1 - 3小时内即可实现。漂白后的组织保存良好，能够耐受进一步的组织化学处理。所有测试的染色和反应所得到的结果与在10%过氧化氢中氧化24 - 48小时后得到的结果相同或更好。
