Cha Yoon Jung, Chang In Ae, Jin Eun-Heui, Song Ji Hye, Hong Jang Hee, Jung Jin-Gyu, Sunwoo Jung
Clinical Trials Center, Chungnam National University Hospital, Daejeon 35015, Republic of Korea.
Department of Medical Science, Chungnam National University College of Medicine, Daejeon 35015, Republic of Korea.
Biomol Ther (Seoul). 2024 Jan 1;32(1):146-153. doi: 10.4062/biomolther.2023.116. Epub 2023 Jul 28.
The (leptin receptor) genotype is associated with obesity. Gut microbiome composition differs between obese and non-obese adults. However, the impact of genotype on gut microbiome composition in humans has not yet been studied. In this study, the association between single nucleotide polymorphism (rs1173100, rs1137101, and rs790419) and the gut microbiome composition in 65 non-obese Korean adults was investigated. Leptin, triglyceride, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol levels were also measured in all participants. Mean ± SD (standard deviation) of age, body mass index, and leptin hormone levels of participants was 35.2 ± 8.1 years, 21.4 ± 1.8 kg/m, and 7989.1 ± 6687.4 pg/mL, respectively. Gut microbiome analysis was performed at the phylum level by 16S rRNA sequencing. Among the 11 phyla detected, only one showed significantly different relative abundances between genotypes. The relative abundance of Candidatus Saccharibacteria was higher in the G/A genotype group than in the G/G genotype group for the rs1137101 single nucleotide polymorphism (=0.0322). Participant characteristics, including body mass index, leptin levels, and other lipid levels, were similar between the rs1137101 G/G and G/A genotypes. In addition, the relative abundances of Fusobacteria and Tenericutes showed significant positive relationship with plasma leptin concentrations (=0.0036 and =0.0000, respectively). In conclusion, genotype and gut microbiome may be associated even in normal-weight Korean adults. However, further studies with a greater number of obese adults are needed to confirm whether genotype is related to gut microbiome composition.
瘦素受体基因型与肥胖相关。肥胖和非肥胖成年人的肠道微生物群组成有所不同。然而，瘦素受体基因型对人类肠道微生物群组成的影响尚未得到研究。在本研究中，调查了65名非肥胖韩国成年人中瘦素受体单核苷酸多态性（rs1173100、rs1137101和rs790419）与肠道微生物群组成之间的关联。所有参与者还测量了瘦素、甘油三酯、低密度脂蛋白胆固醇和高密度脂蛋白胆固醇水平。参与者的年龄、体重指数和瘦素激素水平的平均值±标准差分别为35.2±8.1岁、21.4±1.8kg/m²和7989.1±6687.4pg/mL。通过16S rRNA测序在门水平上进行肠道微生物群分析。在检测到的11个门中，只有一个在瘦素受体基因型之间显示出显著不同的相对丰度。对于rs1137101单核苷酸多态性，G/A基因型组中候选糖菌门的相对丰度高于G/G基因型组（P=0.0322）。rs1137101 G/G和G/A基因型之间的参与者特征，包括体重指数、瘦素水平和其他血脂水平相似。此外，梭杆菌门和柔膜菌门的相对丰度与血浆瘦素浓度呈显著正相关（分别为P=0.0036和P=0.0000）。总之，即使在体重正常的韩国成年人中，瘦素受体基因型和肠道微生物群也可能相关。然而，需要对更多肥胖成年人进行进一步研究，以确认瘦素受体基因型是否与肠道微生物群组成有关。
