State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
Biosensors (Basel). 2023 Jul 19;13(7):746. doi: 10.3390/bios13070746.
As a "gold standard biomarker", cardiac troponin I (cTnI) is widely used to diagnose acute myocardial infarction (AMI). For an early clinical diagnosis of AMI, it is necessary to develop a facile, fast and on-site device for cTnI detection. According to this demand, a point-of-care electrochemical aptasensor was developed for cTnI detection by coupling the advantages of screen-printed carbon electrode (SPCE) with those of an aptamer. Thiol and methylene blue (MB) co-labelled aptamer (MB-Apt-SH) was assembled on the surface of hierarchical flower-like gold nanostructure (HFGNs)-decorated SPCE (SPCE-HFGNs) to recognize and analyze cTnI. In the presence of cTnI, the specific biological recognition reaction between cTnI and aptamer caused the decrease in electrochemical signal. Under the optimal condition, this designed aptasensor showed wide linear range (10 pg/mL-100 ng/mL) and low detection limit for (8.46 pg/mL) for cTnI detection with high selectivity and stability. More importantly, we used a mobile phone coupled with a simple APP to efficiently detect cTnI in 10 μL 100% human serum samples, proving that this aptasensor has a promising potential in point-of-care testing.
作为一种“金标准生物标志物”，心肌肌钙蛋白 I（cTnI）被广泛用于诊断急性心肌梗死（AMI）。为了对 AMI 进行早期临床诊断，有必要开发一种简便、快速、现场的 cTnI 检测设备。根据这一需求，通过将丝网印刷碳电极（SPCE）的优势与适体的优势相结合，开发了一种用于 cTnI 检测的即时电化学适体传感器。巯基和亚甲基蓝（MB）共标记的适体（MB-Apt-SH）被组装在分层花状金纳米结构（HFGNs）修饰的 SPCE（SPCE-HFGNs）表面，以识别和分析 cTnI。在存在 cTnI 的情况下，cTnI 和适体之间的特异性生物识别反应导致电化学信号减少。在最佳条件下，该设计的适体传感器对 cTnI 表现出宽线性范围（10 pg/mL-100 ng/mL）和低检测限（8.46 pg/mL），具有高选择性和稳定性。更重要的是，我们使用智能手机结合简单的应用程序，有效地检测了 10 μL 100%人血清样本中的 cTnI，证明了该适体传感器在即时检测方面具有广阔的应用前景。
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