Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
Biosens Bioelectron. 2022 Sep 15;212:114431. doi: 10.1016/j.bios.2022.114431. Epub 2022 May 28.
The cardiac troponin I (cTnI) detection is increasingly significant given its promising value in the clinical acute myocardial infarction diagnosis. Here a sensitive sandwich-type cTnI electrochemical aptasensor was developed by using zirconium-carbon loaded with Au (Au/Zr-C) as electrode-modified material and snowflake-like PtCuNi catalyst as label material. The Au/Zr-C was prepared from a carbonation process and a reduction step. The PtCuNi was synthesized by a one-pot hydrothermal reaction. On the one hand, due to its many merits of large effective area, rich pores, high degree of graphitization, the assistance of Au, the Au/Zr-C exhibited remarkable electronic conductivity but low catalytical capacity, thus improving the electrochemical property but lowing the background signal of electrode. On the other hand, because of its accessible active sites of the special snowflake-like structure and the synergy of three elements, the PtCuNi catalyst presented excellent catalytic activity and improved stability compared to binary alloy. The recognition reactions were achieved by stepwise incubation of aptamer 1, cTnI, and aptamer 2-PtCuNi (denoted as Apt2-label) on the Au/Zr-C-modified electrode. The electrocatalytic signals of the immobilized Apt2-label towards the HO reduction were recorded in all tests for cTnI analysis. Consequently, this cTnI aptasensor exhibited excellent performance involving a wide linear range of 100 ng mL to 0.01 pg mL with a detection limit of 1.24 × 10 pg mL (S/N = 3), good selectivity, satisfying reproducibility, outstanding stability, and good recovery.
心肌肌钙蛋白 I（cTnI）的检测越来越重要，因为它在临床急性心肌梗死诊断中具有很大的应用价值。在此，我们采用负载 Au 的锆碳（Au/Zr-C）作为电极修饰材料，以及雪花状 PtCuNi 催化剂作为标记材料，开发了一种灵敏的夹心型 cTnI 电化学适体传感器。Au/Zr-C 是通过碳化和还原步骤制备的。PtCuNi 通过一锅水热反应合成。一方面，由于其具有大的有效面积、丰富的孔、高石墨化程度、Au 的辅助作用等优点，Au/Zr-C 表现出显著的电子导电性，但催化能力较低，从而改善了电极的电化学性能，但降低了背景信号。另一方面，由于其特殊的雪花状结构和三种元素的协同作用，PtCuNi 催化剂具有出色的催化活性和稳定性，与二元合金相比有所提高。在 Au/Zr-C 修饰电极上，通过逐步孵育适体 1、cTnI 和适体 2-PtCuNi（表示为 Apt2-label）来实现识别反应。在所有用于 cTnI 分析的测试中，记录了固定化 Apt2-label 对 HO 还原的电催化信号。因此，该 cTnI 适体传感器表现出优异的性能，线性范围为 100ng/mL 至 0.01pg/mL，检测限为 1.24×10pg/mL（S/N=3），具有良好的选择性、重现性、出色的稳定性和良好的回收率。
