Ghazwani Mohammed, Hani Umme, Alqarni Mohammed H, Alam Aftab
Department of Pharmaceutics, College of Pharmacy, King Khalid University, Abha 61441, Saudi Arabia.
Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al Kharj 11942, Saudi Arabia.
Gels. 2023 Jul 6;9(7):550. doi: 10.3390/gels9070550.
This work aimed to overcome the disadvantages of the oral administration of beta-caryophyllene and boost efficiency by developing a nanostructured lipid carrier for topical administration of the drug in skin disorders. The heat emulsification method was utilized to produce beta-caryophyllene-loaded nanostructured lipid carriers. The newly created formulation was examined for its particle size, entrapment efficiency, and zeta potential after being improved using the Box-Behnken Design. The chosen formulation underwent tests to determine its ex vivo skin retention, dermatokinetic, in vitro release, antioxidant, and confocal laser scanning microscopy study. The findings of the characterization of the nanostructured lipid carriers demonstrated that the particles had a spherical form and a size of 210.86 nm (0.263 polydispersity index). The entrapment efficiency was determined to be 86.74%, and the zeta potential was measured to be -26.97 mV. The in vitro release investigation showed that nanostructure lipid carriers were capable of releasing regulated amounts of beta-caryophyllene for up to 24 hrs. In comparison to the traditional gel formulation, the ex vivo investigation demonstrated a 1.94-fold increase in the skin's capacity to retain the substance. According to the findings of the study, nanostructure lipid carriers loaded with beta-caryophyllene have the potential to be investigated for use as a topical administration method in skin disorders with enhanced skin retention and effectiveness.
这项工作旨在克服β-石竹烯口服给药的缺点，并通过开发一种用于皮肤疾病药物局部给药的纳米结构脂质载体来提高效率。采用热乳化法制备了载有β-石竹烯的纳米结构脂质载体。在使用Box-Behnken设计进行改进后，对新制备的制剂进行了粒径、包封率和zeta电位的检测。对所选制剂进行了体外皮肤滞留、皮肤动力学、体外释放、抗氧化和共聚焦激光扫描显微镜研究。纳米结构脂质载体的表征结果表明，颗粒呈球形，粒径为210.86 nm（多分散指数为0.263）。包封率测定为86.74%，zeta电位测定为-26.97 mV。体外释放研究表明，纳米结构脂质载体能够在长达24小时内释放出定量的β-石竹烯。与传统凝胶制剂相比，体外研究表明皮肤保留该物质的能力提高了1.94倍。根据研究结果，载有β-石竹烯的纳米结构脂质载体有潜力作为一种局部给药方法用于皮肤疾病，具有增强的皮肤滞留性和有效性。
