Mehmood Yasir, Shahid Hira, Arshad Numera, Rasul Akhtar, Jamshaid Talha, Jamshaid Muhammad, Jamshaid Usama, Uddin Mohammad N, Kazi Mohsin
Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad P.O. Box 38000, Pakistan.
Riphah Institute of Pharmaceutical Sciences (RIPS), Riphah International University Faisalabad, Faisalabad P.O. Box 38000, Pakistan.
Gels. 2023 Jul 6;9(7):551. doi: 10.3390/gels9070551.
Numerous carbohydrate polymers are frequently used in wound-dressing films because they are highly effective materials for promoting successful wound healing. In this study, we prepared amikacin (AM)-containing hydrogel films through the cross-linking of chitosan (CS) with folic acid along with methacrylic acid (MA), ammonium peroxodisulfate (APS), and methylenebisacrylamide (MBA). In the current studies, an effort has been made to look at the possibilities of these materials in developing new hydrogel film wound dressings meant for a slow release of the antibiotic AM and to enhance the potential for wound healing.
Free-radical polymerization was used to generate the hydrogel film, and different concentrations of the CS polymer were used. Measurements were taken of the film thickness, weight fluctuation, folding resistance, moisture content, and moisture uptake. HPLC, FTIR, SEM, DSC, and AFM analyses were some of the different techniques used to confirm that the films were successfully developed.
The AM release profile demonstrated regulated release over a period of 24 h in simulated wound media at pH 5.5 and 7.4, with a low initial burst release. The antibacterial activity against gram-negative bacterial strains exhibited substantial effectiveness, with inhibitory zones measuring approximately 20.5 ± 0.1 mm. Additionally, in vitro cytocompatibility assessments demonstrated remarkable cell viability, surpassing 80%, specifically when evaluated against human skin fibroblast (HFF-1) cells.
The exciting findings of this study indicate the promising potential for further development and testing of these hydrogel films, offering effective and controlled antibiotic release to enhance the process of wound healing.
许多碳水化合物聚合物常用于伤口敷料薄膜，因为它们是促进伤口成功愈合的高效材料。在本研究中，我们通过壳聚糖（CS）与叶酸以及甲基丙烯酸（MA）、过硫酸铵（APS）和亚甲基双丙烯酰胺（MBA）交联制备了含阿米卡星（AM）的水凝胶薄膜。在当前研究中，已努力探讨这些材料在开发新型水凝胶薄膜伤口敷料方面的可能性，这种敷料旨在缓慢释放抗生素AM并增强伤口愈合潜力。
采用自由基聚合生成水凝胶薄膜，并使用不同浓度的CS聚合物。测量了薄膜厚度、重量波动、抗折性、水分含量和吸水量。HPLC、FTIR、SEM、DSC和AFM分析是用于确认薄膜成功制备的一些不同技术。
AM释放曲线表明，在pH 5.5和7.4的模拟伤口介质中，AM在24小时内呈控释状态，初始突释较低。对革兰氏阴性菌菌株的抗菌活性显示出显著效果，抑菌圈约为20.5±0.1毫米。此外，体外细胞相容性评估显示细胞活力显著，超过80%，特别是在针对人皮肤成纤维细胞（HFF-1）进行评估时。
本研究的令人兴奋的结果表明，这些水凝胶薄膜具有进一步开发和测试的广阔前景，可提供有效且可控的抗生素释放，以增强伤口愈合过程。
