Chen Lusheng, Hao Dazhi, Dou Kai, Lang Bo, Wang Xinhua, Li Yaqian, Chen Jie
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China.
J Fungi (Basel). 2023 Jul 20;9(7):767. doi: 10.3390/jof9070767.
spp. are widely used beneficial microbes in agricultural production; however, the improper carrier choice for agent preparation can alter the effectiveness of fungicides. In this study, the co-culture of four strains produced a large amount of free amino acids, with a content of 392.8414 ug/mL, and significantly improved the production level of γ-aminobutyric acid. A greenhouse experiment further showed that the co-culture of synergistically improved the female flower development and bacterial angular leaf spot resistance. The effects of ten kinds of carriers were compared in terms of water absorption and heat generation, as well as their effects on the seedling emergence rate and the plant growth promotion of maize, cucumber, and pakchoi cabbage. Each carrier was screened to mix with four strains of co-culture metabolites to prepare highly soluble and quality powders. The results showed that there were different effects of the carriers themselves and strain co-culture metabolite powder prepared with the carriers on seedling emergence rate and seedling growth. Β-cyclodextrin performed best in high solubility and low heat generation upon absorbing water and in easy drying in processing operations. strains co-culture metabolite powder with β-cyclodextrin as a carrier provided the most obvious promotion effects on seedling emergence rate and seedling growth. Therefore, β-cyclodextrin was determined to be an ideal carrier to prepare a highly water-soluble agent. Taken together, the study successfully developed a new type of highly soluble powder containing co-culture metabolites that is expected to benefit farming drip irrigation and spraying systems for the promotion of crop growth and disease control.
芽孢杆菌属是农业生产中广泛使用的有益微生物；然而，制剂制备时载体选择不当会改变杀菌剂的效果。在本研究中，四种芽孢杆菌菌株的共培养产生了大量游离氨基酸，含量为392.8414微克/毫升，并显著提高了γ-氨基丁酸的产量水平。温室试验进一步表明，共培养协同改善了雌花发育和对细菌性角斑病的抗性。比较了十种载体在吸水性、产热方面的效果，以及它们对玉米、黄瓜和小白菜出苗率和植株生长促进的影响。筛选每种载体与四种菌株的共培养代谢产物混合，制备高溶解性和优质粉末。结果表明，载体本身以及用载体制备的芽孢杆菌菌株共培养代谢产物粉末对出苗率和幼苗生长有不同影响。β-环糊精在吸水时具有高溶解性、低产热以及加工操作中易于干燥方面表现最佳。以β-环糊精为载体的芽孢杆菌菌株共培养代谢产物粉末对出苗率和幼苗生长的促进作用最为明显。因此，β-环糊精被确定为制备高水溶性制剂的理想载体。综上所述，该研究成功开发了一种新型的含芽孢杆菌共培养代谢产物的高溶解性粉末，有望惠及农业滴灌和喷雾系统，以促进作物生长和病害防治。
