Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098, PR China.
Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098, PR China.
J Hazard Mater. 2023 Oct 5;459:132087. doi: 10.1016/j.jhazmat.2023.132087. Epub 2023 Jul 17.
The extensive use of antibiotics in intertidal mudflat aquaculture area has substantially increased the dissemination risk of antibiotic resistance genes (ARGs). As hosts of ARGs, bacteria and virus exert vital effects on ARG dissemination. However, the insights for the interrelationships among ARGs, bacteria, and virus have not been thoroughly explored in intertidal mudflat. Therefore, this study attempts to unravel the occurrence, dissemination, evolution, and driving mechanisms of ARGs associated with bacterial and viral communities using metagenomic sequencing in a typical intertidal mudflat. Abundant and diverse ARGs (22 types and 437 subtypes) were identified and those of ARGs were higher in spring than in autumn. It is worthy noted that virus occupied a more essential position than bacteria for ARGs dissemination through network analysis. Meanwhile, nitrogen exerted indirect effect on ARG profiles by shaping viral and bacterial diversity. According to the results of neutral and null models, deterministic processes dominated the ARG community assembly by controlling sediment nitrogen and antibiotics. Homogeneous and variable selection dominated phylogenetic turnover of ARG community, contributing 46.15% and 45.90% of the total processes, respectively. This study can hence theoretically support for the ARG pollution control and management in intertidal mudflat aquaculture area.
抗生素在潮间带泥滩水产养殖区的广泛使用，极大地增加了抗生素耐药基因（ARGs）的传播风险。作为 ARGs 的宿主，细菌和病毒对 ARG 的传播起着至关重要的作用。然而，在潮间带泥滩中，人们对 ARGs、细菌和病毒之间的相互关系的认识还不够深入。因此，本研究试图通过在典型的潮间带泥滩中进行宏基因组测序，揭示与细菌和病毒群落相关的 ARGs 的发生、传播、进化和驱动机制。研究结果表明，大量且多样的 ARGs（22 种和 437 种亚型）被鉴定出来，并且 ARGs 的数量在春季高于秋季。值得注意的是，通过网络分析，病毒在 ARGs 的传播中比细菌占据更重要的地位。同时，氮通过塑造病毒和细菌的多样性对 ARG 图谱施加间接影响。根据中性和零模型的结果，确定性过程通过控制沉积物氮和抗生素来主导 ARG 群落组装。同质和可变选择主导了 ARG 群落的系统发育周转率，分别贡献了总过程的 46.15%和 45.90%。因此，本研究从理论上支持了潮间带泥滩水产养殖区的 ARG 污染控制和管理。
