State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
State Key Joint Laboratory of Environment Simulation and Pollution Control (SKJLESPC), Beijing Key Laboratory for Emerging Organic Contaminants Control (BKLEOC), School of Environment, POPs Research Center, Tsinghua University, Beijing 100084, China.
J Environ Sci (China). 2022 Mar;113:345-355. doi: 10.1016/j.jes.2021.06.026. Epub 2021 Jul 7.
To meet the rapidly growing global demand for aquaculture products, large amounts of antibiotics were used in aquaculture, which might accelerate the evolution of antibiotic-resistant bacteria (ARB) and the propagation of antibiotic genes (ARGs). In our research, we revealed the ARGs profiles, their co-occurrence with mobile genetic elements (MGEs), and potential hosts in sediments of a crab pond wastewater purification system based on metagenomic analysis. The residual antibiotic seems to increase the propagation of ARGs in the crab pond, but there was no clear relationship between a given antibiotic type and the corresponding resistance genes. The effect of aquaculture on sediment was not as profound as that of other anthropogentic activities, but increased the relative abundance of sulfonamide resistance gene. A higher abundance of MGEs, especially plasmid, increased the potential ARGs dissemination risk in crab and purification ponds. Multidrug and sulfonamide resistance genes had greater potential to transfer because they were more frequently carried by MGEs. The horizontal gene transfer was likely to occur among a variety of microorganisms, and various ARGs hosts including Pseudomonas, Acinetobacter, Escherichia, and Klebsiella were identified. Bacterial community influenced the composition of ARG hosts, and Proteobacteria was the predominant hosts. Overall, our study provides novel insights into the environmental risk of ARGs in sediments of aquaculture wastewater treatment system.
为满足全球对水产养殖产品不断增长的需求，水产养殖中大量使用抗生素，这可能加速抗生素耐药细菌（ARB）的进化和抗生素基因（ARGs）的传播。在我们的研究中，我们通过宏基因组分析揭示了基于蟹塘废水净化系统沉积物中 ARGs 图谱、它们与移动遗传元件（MGEs）的共同出现以及潜在宿主的关系。残留抗生素似乎增加了蟹塘中 ARGs 的传播，但给定抗生素类型与相应的耐药基因之间没有明显的关系。水产养殖对沉积物的影响不如其他人为活动那样深远，但增加了磺胺类耐药基因的相对丰度。MGEs（特别是质粒）的丰度增加，增加了蟹塘和净化塘中 ARGs 传播的潜在风险。由于 MGEs 更频繁地携带多药和磺胺类耐药基因，因此这些基因具有更大的转移潜力。水平基因转移可能发生在各种微生物之间，并且鉴定出了包括 Pseudomonas、Acinetobacter、Escherichia 和 Klebsiella 在内的各种 ARG 宿主。细菌群落影响 ARG 宿主的组成，变形菌门是主要的宿主。总的来说，我们的研究为水产养殖废水处理系统沉积物中 ARGs 的环境风险提供了新的见解。
