Department of Chemistry, Stanford University, Stanford, California 94305, United States.
Department of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, California 94305, United States.
ACS Sens. 2023 Aug 25;8(8):3264-3271. doi: 10.1021/acssensors.3c01345. Epub 2023 Jul 28.
The rapid and accurate detection of bacteria resistance to β-lactam antibiotics is critical to inform optimal treatment and prevent overprescription of potent antibiotics. Here, we present a fast, culture-independent method for the detection of extended-spectrum β-lactamases (ESBLs) using surface-enhanced Raman scattering (SERS). The method uses Raman probes that release sulfur-based Raman active molecules in the presence of β-lactamases. The released thiol molecules can be captured by gold nanoparticles, leading to amplified Raman signals. A broad-spectrum cephalosporin probe R1G and an ESBL-specific probe R3G are designed to enable duplex detection of bacteria expressing broad-spectrum β-lactamases or ESBLs with a detection limit of 10 cfu/mL in 1 h incubation. Combined with a portable Raman microscope, our culturing-free SERS assay has reduced screening time to 1.5 h without compromising sensitivity and specificity.
快速准确地检测细菌对β-内酰胺类抗生素的耐药性对于指导最佳治疗方案和防止强效抗生素的过度处方至关重要。在这里，我们提出了一种使用表面增强拉曼散射（SERS）快速、无需培养的方法来检测超广谱β-内酰胺酶（ESBLs）。该方法使用拉曼探针，在β-内酰胺酶存在的情况下释放基于硫的拉曼活性分子。释放的巯基分子可以被金纳米粒子捕获，从而导致拉曼信号增强。设计了一种广谱头孢菌素探针 R1G 和一种 ESBL 特异性探针 R3G，以实现对表达广谱β-内酰胺酶或 ESBL 的细菌进行双检测，在 1 小时孵育时间内的检测限为 10 cfu/mL。结合便携式拉曼显微镜，我们无需培养的 SERS 测定法将筛选时间缩短至 1.5 小时，而不会影响灵敏度和特异性。
