Ni Jialing, Ji Jiayuan, Li Yu-You, Kubota Kengo
Department of Civil and Environmental Engineering, Tohoku University, 6-6-06 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579 Japan; Department of Frontier Sciences for Advanced Environment, Tohoku University, 6-6-06 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579 Japan.
Department of Civil and Environmental Engineering, Tohoku University, 6-6-06 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579 Japan; Institute for Future Initiatives, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8654, Japan.
Bioresour Technol. 2023 Nov;387:129564. doi: 10.1016/j.biortech.2023.129564. Epub 2023 Jul 26.
An anaerobic membrane bioreactor (AnMBR) treated domestic sewage at 15 °C under different hydraulic retention time (HRT) conditions (6, 12, 16, and 24 h). Propidium monoazide (PMA)-PCR excluded microorganisms without intact cell membranes, focusing on the viable microbial community in anaerobic digestion. The results showed that the 6-hour HRT had poor treatment performance: low chemical oxygen demand removal efficiency (below 80%) and high mean trans-membrane pressure and flux (15 kPa and 9.4 L/(m h)). Comparatively, PMA-PCR combined with next-generation sequencing improved the identification of microbial changes compared to conventional 16S rRNA gene sequencing. HRT influenced microorganisms in the hydrolysis and acid-production stages, including carbohydrate-degrading bacteria such as Bifidobacterium and Prevotella 1. Remarkably, a comparison with an AnMBR at 25 °C showed Proteobacteria to be the main cause of membrane fouling in the low-temperature AnMBR, with most operational taxonomic units negatively correlated with HRT and solids retention time.
一个厌氧膜生物反应器（AnMBR）在15℃下于不同水力停留时间（HRT）条件（6、12、16和24小时）处理生活污水。单叠氮化丙锭（PMA）-PCR排除了没有完整细胞膜的微生物，专注于厌氧消化中的活微生物群落。结果表明，6小时的HRT处理性能较差：化学需氧量去除效率低（低于80%），平均跨膜压力和通量高（15 kPa和9.4 L/(m·h)）。相比之下，与传统的16S rRNA基因测序相比，PMA-PCR结合下一代测序改进了对微生物变化的识别。HRT影响水解和产酸阶段的微生物，包括双歧杆菌和普雷沃氏菌属1等碳水化合物降解细菌。值得注意的是，与25℃下的AnMBR比较表明，变形菌是低温AnMBR中膜污染的主要原因，大多数操作分类单元与HRT和固体停留时间呈负相关。
