Planta Piloto de Procesos Industriales Microbiológicos (PROIMI-CONICET), Av. Belgrano y Pje. Caseros, San Miguel de Tucumán (T4001MVB), Tucumán, Argentina.
YPF Tecnología (Y-TEC), Av. del Petróleo Argentino (RP10) S/N entre 129 y 143 (1923), Berisso, Buenos Aires, Argentina.
Bioresour Technol. 2023 Nov;387:129582. doi: 10.1016/j.biortech.2023.129582. Epub 2023 Jul 26.
Dibenzothiophene (DBT), which belongs to the group of polycyclic aromatic heterocycles of sulfur, is a model substance to study the removal of sulfur compounds from oil due to its recalcitrance to traditional and specific removal processes. The aim of this work was to evaluate DBT bioremoval by environmental bacteria and its relationship with polyphosphate (polyP) accumulation, cell surface characteristics and bioemulsifying activity. Pseudomonas sp. P26 achieved the highest DBT removal percentage (48%) after 7 days of incubation. Moreover, positive correlations were estimated between DBT removal and bioemulsifying activity and biofilm formation. A strain-dependent relationship between the content of intracellular polyP and the presence of DBT in the culture medium was also demonstrated. The study of these bacterial characteristics, which could promote DBT transformation, is a first approach to select DBT-removing bacteria, in order to develop bioformulations that are able to contribute to desulfurization processes of petroleum-derived pollutants in the future.
二苯并噻吩（DBT）属于多环芳烃杂环硫族，是研究由于其对传统和特定去除工艺的抗性而从油中去除硫化合物的模型物质。本工作的目的是评估环境细菌对 DBT 的生物去除及其与多聚磷酸盐（polyP）积累、细胞表面特性和生物乳化活性的关系。经过 7 天的培养，假单胞菌 P26 实现了最高的 DBT 去除率（48%）。此外，还估计了 DBT 去除与生物乳化活性和生物膜形成之间的正相关关系。还证明了细胞内多聚磷酸盐的含量与培养基中 DBT 的存在之间存在菌株依赖性关系。研究这些能够促进 DBT 转化的细菌特性是选择 DBT 去除细菌的第一步，以便未来能够开发出能够有助于石油衍生污染物脱硫过程的生物制剂。
