Kumar Ravinder, Lal Milan Kumar, Tiwari Rahul Kumar, Chourasia Kumar Nishant, Kumar Awadhesh, Kumar Rakesh, Sharma Shivangi, Singh Brajesh
ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh, India.
ICAR-Central Research Institute for Jute and Allied Fibres, Barrackpore 700121, West Bengal, India.
Antioxidants (Basel). 2023 Jul 18;12(7):1447. doi: 10.3390/antiox12071447.
The potato apical leaf curl disease is caused by tomato leaf curl New Delhi virus-potato (ToLCNDV-potato), which severely alters a plant's starch metabolism, starch hydrolysing enzymes, and antioxidant mechanism. In this study, the result suggested that ToLCNDV-potato significantly ( < 0.01) affected the morphological parameters and photosynthetic pigment system in both the cultivars of potato, viz., Kufri Pukhraj (susceptible) and Kufri Bahar (tolerant). However, the impact of ToLCNDV-potato was lower in Kufri Bahar. Moreover, the viral infection in potato showed significant ( < 0.01) enhancement in the leakage of plant oxidative metabolites such as proline and malondialdehyde (MDA) which was further confirmed with higher electrolyte leakage. The viral infection imbalance of starch metabolism in the leaves ultimately affects the carbohydrate profile. ToLCNDV-potato significantly lowered starch synthesis, enhanced the accumulation of sucrose, glucose, fructose and-which was further validated by enzymatic estimation of β-amylase-α-amylase and phosphorylase activity in the leaves of both cultivars. The antioxidant enzymes, viz., catalase, ascorbate peroxidase, and superoxide dismutase, were reported to be enhanced in both the cultivars due to ToLCNDV-potato infection. The higher enhancement of antioxidant enzyme activity was observed in Kufri Bahar, which signifies its resistant attributes. These findings in the potato plant broaden our understanding of the regulatory mechanisms of starch metabolism and antioxidant activity and provide proof of concept for breeding potato for ToLCNDV-potato tolerance.
马铃薯顶端叶片卷曲病由番茄卷叶新德里病毒-马铃薯株系（ToLCNDV-马铃薯）引起，该病毒会严重改变植物的淀粉代谢、淀粉水解酶和抗氧化机制。在本研究中，结果表明ToLCNDV-马铃薯对两个马铃薯品种，即库弗里·普赫拉杰（易感品种）和库弗里·巴哈尔（耐病品种）的形态参数和光合色素系统均有显著影响（<0.01）。然而，ToLCNDV-马铃薯对库弗里·巴哈尔的影响较小。此外，马铃薯中的病毒感染显著（<0.01）增加了植物氧化代谢产物如脯氨酸和丙二醛（MDA）的渗漏，更高的电解质渗漏进一步证实了这一点。叶片中淀粉代谢的病毒感染失衡最终会影响碳水化合物分布。ToLCNDV-马铃薯显著降低了淀粉合成，增加了蔗糖、葡萄糖、果糖的积累，这通过对两个品种叶片中β-淀粉酶、α-淀粉酶和磷酸化酶活性的酶学测定得到了进一步验证。据报道，由于ToLCNDV-马铃薯感染，两个品种中的抗氧化酶，即过氧化氢酶、抗坏血酸过氧化物酶和超氧化物歧化酶均有所增强。在库弗里·巴哈尔中观察到抗氧化酶活性的增强更为显著，这表明了其抗性特征。马铃薯植株中的这些发现拓宽了我们对淀粉代谢和抗氧化活性调控机制的理解，并为培育耐ToLCNDV-马铃薯的马铃薯品种提供了概念验证。
