Sharma Namisha, Muthamilarasan Mehanathan, Dulani Priya, Prasad Manoj
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, Telangana, India.
Genomics. 2021 May;113(3):889-899. doi: 10.1016/j.ygeno.2021.01.022. Epub 2021 Jan 30.
In the present study, genes encoding for six major classes of enzymatic antioxidants, namely superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), Peroxidase (Prx) and glutathione S-transferase (GST) are identified in tomato. Their expression was studied in tomato cultivars contrastingly tolerant to ToLCNDV during virus infection and different hormone treatments. Significant upregulation of SlGR3, SlPrx25, SlPrx75, SlPrx95, SlGST44, and SlGST96 was observed in the tolerant cultivar during disease infection. Virus-induced gene silencing of SlGR3 in the tolerant cultivar conferred disease susceptibility to the knock-down line, and higher accumulation (80%) of viral DNA was observed in the tolerant cultivar. Further, subcellular localization of SlGR3 showed its presence in cytoplasm, and its enzymatic activity was found to be increased (65%) during ToLCNDV infection. Knock-down lines showed ~3- and 3.5-fold reduction in GR activity, which altogether underlines that SlGR3 is vital component of the defense mechanism against ToLCNDV infection.
在本研究中，在番茄中鉴定出了编码六大类酶促抗氧化剂的基因，即超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）、过氧化物酶（Prx）和谷胱甘肽S-转移酶（GST）。研究了它们在病毒感染和不同激素处理期间对番茄黄化曲叶病毒（ToLCNDV）具有不同耐受性的番茄品种中的表达情况。在抗病品种中，观察到SlGR3、SlPrx25、SlPrx75、SlPrx95、SlGST44和SlGST96在病害感染期间显著上调。在抗病品种中对SlGR3进行病毒诱导的基因沉默，使敲除系对病害敏感，并且在抗病品种中观察到病毒DNA积累增加（约80%）。此外，SlGR3的亚细胞定位显示其存在于细胞质中，并且在ToLCNDV感染期间其酶活性增加（约65%）。敲除系的GR活性降低了约3倍和3.5倍，这共同表明SlGR3是抵御ToLCNDV感染的防御机制的重要组成部分。
