Selionova Marina I, Aibazov Magomet M, Zharkova Ekaterina K
Subdepartment of Animal Breeding, Genetics and Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, Timiryazevskaya Street, 41, 127343 Moscow, Russia.
North Caucasian Agrarian Center, Zootechnicheski 15, 355017 Stavropol, Russia.
Animals (Basel). 2023 Jul 20;13(14):2361. doi: 10.3390/ani13142361.
This article presents data from experiments to determine the cryoresistance of Charollais sheep embryos, depending on the stage of embryo development and the method of freezing, as well as the results of embryo transfer. The study design consisted of a study on the cryopreservation of ewe embryos at different developmental stages (early, 2-8 blastomeric and late, at the morula/blastocyst stage), two cryopreservation protocols (slow freezing and ultra-fast vitrification), and embryo transfer of cryo- and fresh embryos. Embryos from Charollais sheep donors (n = 12) were recovered after induction of superovulation. The embryos were recovered surgically (laparotomy) on days 2 and 6 after insemination. Before there was transfer to recipients, part of embryos was cryopreserved using standard slow freezing and ultra-fast vitrification methods. The average ovarian response was 7.54 ovulations per donor, and 5.83 embryos per donor were collected. No effect of the cryopreservation method and embryo development stage on the preservation of the morphological structure of embryos was found. There were no significant differences in the survival rate of cryoembryos at different development stages, frozen using different techniques, and after transfer to recipients. Differences in cryoresistance between embryonic developmental stages in favor of the morula/blastocyst stage were found (survival after thawing 86.4% vs. 75.0% in early embryos). At different stages of development, the survival rate of fresh embryos (45.8%) compared to cryopreserved ones (30.2%) was significantly higher ( < 0.05), while among fresh ones, the best survival rate (50.0%) was observed after the transfer of morules and blastocysts.
本文介绍了一些实验数据，这些实验旨在确定夏洛莱羊胚胎的抗冻性，该抗冻性取决于胚胎发育阶段、冷冻方法以及胚胎移植的结果。研究设计包括对处于不同发育阶段（早期，2 - 8细胞期和晚期，桑葚胚/囊胚期）的母羊胚胎进行冷冻保存的研究、两种冷冻保存方案（慢速冷冻和超快速玻璃化）以及冷冻胚胎和新鲜胚胎的移植。对12只夏洛莱羊供体进行超数排卵诱导后收集胚胎。在授精后第2天和第6天通过手术（剖腹术）回收胚胎。在将胚胎移植到受体之前，部分胚胎使用标准慢速冷冻和超快速玻璃化方法进行冷冻保存。每个供体的平均卵巢反应为7.54个排卵，每个供体收集到5.83个胚胎。未发现冷冻保存方法和胚胎发育阶段对胚胎形态结构保存有影响。不同发育阶段的冷冻胚胎在使用不同技术冷冻并移植到受体后，其存活率没有显著差异。发现胚胎发育阶段之间的抗冻性存在差异，桑葚胚/囊胚期更具优势（解冻后存活率早期胚胎为75.0%，桑葚胚/囊胚期为86.4%）。在不同发育阶段，新鲜胚胎的存活率（45.8%）显著高于冷冻胚胎（30.2%）（<0.05），而在新鲜胚胎中，桑葚胚和囊胚移植后的存活率最高（50.0%）。
