Pruss Agata, Kwiatkowski Paweł, Sienkiewicz Monika, Masiuk Helena, Łapińska Agnieszka, Kot Barbara, Kilczewska Zuzanna, Giedrys-Kalemba Stefania, Dołęgowska Barbara
Department of Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland.
Department of Diagnostic Immunology, Pomeranian Medical University in Szczecin, Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland.
Antibiotics (Basel). 2023 Jul 24;12(7):1224. doi: 10.3390/antibiotics12071224.
is an important opportunistic pathogen responsible for severe infections, mainly urinary tract infections (UTIs) and pneumonia. Hospital epidemic infections caused by multiresistant strains of carbapenemase-producing are the most concerning. NDM-producing strains are resistant to a wide range of antibiotics and have become the most significant threat. Determining the natural reservoirs and routes of infections is essential to end hospital outbreaks. Understanding the relatedness of strains is essential to determine the range and nature of the infection. The study compared phylogenetic relatedness between multiresistant strains isolated from hospitalized patients. Susceptibility to drugs and mechanisms of resistance were confirmed using phenotypic methods. PFGE was used to analyze the relatedness between strains. We analyzed 69 strains from various healthcare units. The isolates were mainly identified from urine. Strains were resistant to β-lactam antibiotics with β-lactamase inhibitors, cephalosporins, and quinolones. Their susceptibility to aminoglycosides and carbapenem antibiotics was diverse. Most of the isolated strains produced New Delhi metallo-ß-lactamase (NDM). Although strains were classified into several genotype clusters, closely related isolates were confirmed in the same hospital's wards, and in two hospitals in the same province.
是一种重要的机会致病菌，可导致严重感染，主要是尿路感染（UTIs）和肺炎。由产碳青霉烯酶的多重耐药菌株引起的医院感染流行最为令人担忧。产NDM菌株对多种抗生素耐药，已成为最重大的威胁。确定自然宿主和感染途径对于终结医院感染暴发至关重要。了解菌株的相关性对于确定感染的范围和性质至关重要。该研究比较了从住院患者中分离出的多重耐药菌株之间的系统发育相关性。使用表型方法确认了对药物的敏感性和耐药机制。脉冲场凝胶电泳（PFGE）用于分析菌株之间的相关性。我们分析了来自各个医疗单位的69株菌株。分离株主要从尿液中鉴定出来。菌株对含β-内酰胺酶抑制剂的β-内酰胺类抗生素、头孢菌素和喹诺酮类耐药。它们对氨基糖苷类和碳青霉烯类抗生素的敏感性各不相同。大多数分离株产生新德里金属β-内酰胺酶（NDM）。尽管菌株被分为几个基因型簇，但在同一家医院的病房以及同一省份的两家医院中确认了密切相关的分离株。
