Moorkens Kerlijne, Leroy Jo L M R, Quanico Jusal, Baggerman Geert, Marei Waleed F A
Gamete Research Centre, Laboratory for Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, 2610 Wilrijk, Belgium.
Centre for Proteomics, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium.
Biology (Basel). 2023 Jul 17;12(7):1016. doi: 10.3390/biology12071016.
We investigated whether a high-fat/high-sugar (HF/HS) diet alters the lipidomic profile of the oviductal epithelium (OE) and studied the patterns of these changes over time. Female outbred Swiss mice were fed either a control (10% fat) or HF/HS (60% fat, 20% fructose) diet. Mice ( = 3 per treatment per time point) were sacrificed and oviducts were collected at 3 days and 1, 4, 8, 12 and 16 weeks on the diet. Lipids in the OE were imaged using matrix-assisted laser desorption ionisation mass spectrometry imaging. Discriminative / values and differentially regulated lipids were determined in the HF/HS versus control OEs at each time point. Feeding the obesogenic diet resulted in acute changes in the lipid profile in the OE already after 3 days, and thus even before the development of an obese phenotype. The changes in the lipid profile of the OE progressively increased and became more persistent after long-term HF/HS diet feeding. Functional annotation revealed a differential abundance of phospholipids, sphingomyelins and lysophospholipids in particular. These alterations appear to be not only caused by the direct accumulation of the excess circulating dietary fat but also a reduction in the de novo synthesis of several lipid classes, due to oxidative stress and endoplasmic reticulum dysfunction. The described diet-induced lipidomic changes suggest alterations in the OE functions and the oviductal microenvironment which may impact crucial reproductive events that take place in the oviduct, such as fertilization and early embryo development.
我们研究了高脂/高糖（HF/HS）饮食是否会改变输卵管上皮（OE）的脂质组学特征，并研究了这些变化随时间的模式。将远交系瑞士雌性小鼠分为对照组（10%脂肪）或HF/HS组（60%脂肪，20%果糖）进行喂养。在饮食干预后的第3天以及第1、4、8、12和16周，处死小鼠（每个处理组每个时间点n = 3）并收集输卵管。使用基质辅助激光解吸电离质谱成像对OE中的脂质进行成像。在每个时间点，测定HF/HS组与对照组OE之间的判别性p值和差异调节的脂质。喂食致肥胖饮食3天后，OE中的脂质谱就已出现急性变化，甚至在肥胖表型出现之前。长期喂食HF/HS饮食后，OE脂质谱的变化逐渐增加且变得更加持久。功能注释显示，磷脂、鞘磷脂和溶血磷脂的丰度存在差异。这些变化似乎不仅是由于循环中过量膳食脂肪的直接积累，还由于氧化应激和内质网功能障碍导致几种脂质类别的从头合成减少。所述饮食诱导的脂质组学变化表明OE功能和输卵管微环境发生了改变，这可能会影响在输卵管中发生的关键生殖事件，如受精和早期胚胎发育。
