CircAFF1是一种在肺泡横纹肌肉瘤细胞迁移中起作用的环状RNA。

Centrón-Broco Alvaro, Rossi Francesca, Grelloni Chiara, Garraffo Raffaele, Dattilo Dario, Giuliani Andrea, Di Timoteo Gaia, Colantoni Alessio, Bozzoni Irene, Beltran Nebot Manuel

Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, 00185 Rome, Italy.

The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK.

Biomedicines. 2023 Jul 4;11(7):1893. doi: 10.3390/biomedicines11071893.

Circular RNAs (circRNAs), covalently closed RNAs that originate from back-splicing events, participate in the control of several processes, including those that occur in the development of pathological conditions such as cancer. Hereby, we describe circAFF1, a circular RNA overexpressed in alveolar rhabdomyosarcoma. Using RH4 and RH30 cell lines, a classical cell line models for alveolar rhabdomyosarcoma, we demonstrated that circAFF1 is a cytoplasmatic circRNA and its depletion impacts cell homeostasis favouring cell migration through the downregulation of genes involved in cell adhesion pathways. The presented data underline the importance of this circular RNA as a new partial suppressor of the alveolar rhabdomyosarcoma tumour progression and as a putative future therapeutic target.

环状RNA（circRNAs）是源自反向剪接事件的共价闭合RNA，参与多种过程的调控，包括那些发生在诸如癌症等病理状况发展过程中的过程。在此，我们描述了circAFF1，一种在肺泡横纹肌肉瘤中过表达的环状RNA。使用RH4和RH30细胞系（肺泡横纹肌肉瘤的经典细胞系模型），我们证明circAFF1是一种细胞质环状RNA，其缺失影响细胞稳态，通过下调参与细胞粘附途径的基因促进细胞迁移。所呈现的数据强调了这种环状RNA作为肺泡横纹肌肉瘤肿瘤进展的新的部分抑制因子以及作为未来潜在治疗靶点的重要性。