Yang Qiya, Zhang Xi, Solairaj Dhanasekaran, Lin Rouling, Wang Kaili, Zhang Hongyin
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Foods. 2023 Jul 8;12(14):2637. doi: 10.3390/foods12142637.
Studies on the molecular mechanism of antagonistic yeasts to control apple postharvest diseases are not comprehensive enough. Our preliminary investigations screened the biocontrol effect of , an antagonistic yeast, and discovered its control efficacy on apple blue mold decay. However, the molecular mechanism of -induced resistance in apple has not been studied. In this study, proteins from apple treated with and sterile saline were analyzed using TMT proteomics technology. It was found that treatment induced the expressions of apple resistance-related proteins. Among the proteins in -induced apple, proteins related to plant defense mechanisms, such as reactive oxygen species scavenging, improvement of plant resistance and synthesis of resistant substances, improvement of plant disease resistance, the degradation of the pathogen cell wall, cell signaling, antibacterial activity, transport of defense-related substances, and protein processing, were differentially regulated. The results of this study revealed the underlying molecular mechanisms of -induced apple resistance at the protein level; the results also provided a theoretical basis for the commercial application of .
拮抗酵母防治苹果采后病害的分子机制研究还不够全面。我们的初步研究筛选了拮抗酵母的生防效果，并发现了其对苹果青霉腐烂病的防治效果。然而，该拮抗酵母诱导苹果产生抗性的分子机制尚未得到研究。在本研究中，利用TMT蛋白质组学技术分析了用该拮抗酵母和无菌盐水处理的苹果中的蛋白质。结果发现，该拮抗酵母处理诱导了苹果抗性相关蛋白的表达。在该拮抗酵母诱导处理的苹果中的蛋白质中，与植物防御机制相关的蛋白质，如活性氧清除、植物抗性提高、抗性物质合成、植物抗病性提高、病原菌细胞壁降解、细胞信号传导、抗菌活性、防御相关物质运输以及蛋白质加工等，均受到差异调节。本研究结果揭示了该拮抗酵母诱导苹果产生抗性在蛋白质水平上的潜在分子机制；研究结果也为该拮抗酵母的商业应用提供了理论依据。
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