对产志贺毒素的O157:H7有活性的产大肠杆菌素菌株的分子和遗传特征分析

Molecular and Genetic Characterization of Colicinogenic Strains Active against Shiga Toxin-Producing O157:H7.

作者信息

García Mauro D, Ruiz María J, Medina Luis M, Vidal Roberto, Padola Nora L, Etcheverria Analía I

机构信息

Laboratorio de Inmunoquímica y Biotecnología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, CICPBA, Facultad de Ciencias Veterinarias, UNICEN-Campus Universitario, Tandil B7000, Argentina.

Food Science and Technology Department, Faculty of Veterinary Medicine, Universidad de Cordoba, 14071 Córdoba, Spain.

出版信息

Foods. 2023 Jul 11;12(14):2676. doi: 10.3390/foods12142676.

DOI:10.3390/foods12142676

PMID:37509768

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10378606/

Abstract

The objective of this work was to molecularly and genotypically characterize and test the inhibitory activity of six colicinogenic strains (ColEc) and their partially purified colicins against STEC O157:H7 isolated from clinical human cases. Inhibition tests demonstrated the activity of these strains and their colicins against STEC O157:H7. By PCR it was possible to detect colicins Ia, E7, and B and microcins M, H47, C7, and J25. By genome sequencing of two selected ColEc strains, it was possible to identify additional colicins such as E1 and Ib. No genes coding for and were detected after analyzing the genome sequence. The inhibitory activity of ColEc against STEC O157:H7 used as an indicator showed that colicins are potent growth inhibitors of O157:H7, being a potential alternative to reduce the presence of pathogens of public health relevance.

摘要

这项工作的目的是对六种产大肠杆菌素菌株（ColEc）及其部分纯化的大肠杆菌素进行分子和基因型特征分析，并测试它们对从临床人类病例中分离出的肠出血性大肠杆菌O157:H7的抑制活性。抑制试验证明了这些菌株及其大肠杆菌素对肠出血性大肠杆菌O157:H7的活性。通过聚合酶链反应（PCR）能够检测到大肠杆菌素Ia、E7和B以及微菌素M、H47、C7和J25。通过对两个选定的ColEc菌株进行基因组测序，能够鉴定出其他大肠杆菌素，如E1和Ib。在分析基因组序列后，未检测到编码和的基因。以肠出血性大肠杆菌O157:H7作为指示菌，ColEc对其的抑制活性表明，大肠杆菌素是O157:H7的有效生长抑制剂，是减少具有公共卫生相关性病原体存在的潜在替代方法。