Crescente Giuseppina, Cascone Giovanni, Petruzziello Antonio, Bouymajane Aziz, Volpe Maria Grazia, Russo Gian Luigi, Moccia Stefania
National Research Council, Institute of Food Sciences, 83100 Avellino, Italy.
National Research Council, Research Institute on Terrestrial Ecosystems, 80131 Napoli, Italy.
Foods. 2023 Jul 11;12(14):2678. doi: 10.3390/foods12142678.
The wine industry produces large amounts of grape pomace (GP), a waste that needs to be disposed of properly. Bioactive compounds with high added value can be recovered from GP as an interesting strategy to reduce the environmental impact. Here, two different technologies were employed to recover polyphenol compounds from GP: microwave hydrodiffusion and gravity (MHG) and ultrasound-assisted extraction (UAE). The further purification of UAE and MHG extracts was carried out through solid-phase extraction (SPE) to obtain three fractions, F1, F2 and F3. ATR-FTIR analysis confirmed the presence of sugar and polysaccharide components in F1, as well as non-anthocyanin and anthocyanin compounds in F2 and F3, respectively. Also, the chemical profile was determined by HPLC-UV-DAD, identifying the presence of catechin in F2, and malvidin-3--glucoside chloride and cyanidin chloride derivative as the main anthocyanin compounds in F3. The fractions and their parental extracts were characterized for total phenolic content (TPC) and scavenger activity by assays. We found that F2-MHG and F3-MHG contained phenol contents 6.5 and 8.5 times higher than those of the parental non-fractionated extracts. Finally, F3-MHG (100 μg/mL, /) was shown to reduce the proliferation of HT-29 cells.
葡萄酒行业会产生大量葡萄皮渣（GP），这是一种需要妥善处理的废弃物。从葡萄皮渣中回收具有高附加值的生物活性化合物是减少环境影响的一项有趣策略。在此，采用了两种不同技术从葡萄皮渣中回收多酚化合物：微波水解扩散与重力法（MHG）和超声辅助提取法（UAE）。通过固相萃取（SPE）对UAE和MHG提取物进行进一步纯化，以获得三个组分，F1、F2和F3。衰减全反射傅里叶变换红外光谱（ATR-FTIR）分析证实F1中存在糖和多糖成分，F2和F3中分别存在非花青素和花青素化合物。此外，通过高效液相色谱-紫外-二极管阵列检测（HPLC-UV-DAD）确定化学图谱，鉴定出F2中存在儿茶素，F3中主要的花青素化合物为氯化锦葵素-3-O-葡萄糖苷和氯化矢车菊素衍生物。通过测定对各组分及其原始提取物的总酚含量（TPC）和清除活性进行了表征。我们发现F2-MHG和F3-MHG的酚含量分别比未分级的原始提取物高6.5倍和8.5倍。最后，F3-MHG（100μg/mL，/）显示出可降低HT-29细胞的增殖。
