Institute of Food Sciences, National Research Council, 83100 Avellino, Italy.
Nutrients. 2022 Dec 30;15(1):182. doi: 10.3390/nu15010182.
Regular consumption of olive oil is associated with protection against chronic-degenerative diseases, such as cancer. Epidemiological evidence indicates an inverse association between olive oil intake and bladder cancer risk. Bladder cancer is among the most common forms of cancer; in particular, the transitional cell carcinoma histotype shows aggressive behavior. We investigated the anti-proliferative effects of a phenolic extract prepared from an extra virgin olive oil (EVOOE) on two human bladder cancer cell lines, namely RT112 and J82, representing the progression from low-grade to high-grade tumors, respectively. In RT112, the EVOOE reduced cell viability (IC50 = 240 μg/mL at 24 h), triggering a non-protective form of autophagy, evidenced by the autophagosome formation and the increase in LC-3 lipidation. In J82, EVOOE induced a strong decrease in cell viability after 24 h of treatment (IC50 = 65.8 μg/mL) through rapid and massive apoptosis, assessed by Annexin V positivity and caspase-3 and -9 activation. Moreover, in both bladder cancer cell lines, EVOOE reduced intracellular reactive oxygen species, but this antioxidant effect was not correlated with its anti-proliferative outcomes. Data obtained suggest that the mixture of phenolic compounds in extra virgin olive oil activates different anti-proliferative pathways.
橄榄油的常规消费与预防慢性退行性疾病（如癌症）有关。流行病学证据表明，橄榄油的摄入量与膀胱癌风险呈负相关。膀胱癌是最常见的癌症之一；特别是移行细胞癌组织类型表现出侵袭性行为。我们研究了一种从特级初榨橄榄油（EVOOE）中制备的酚类提取物对两种人膀胱癌细胞系 RT112 和 J82 的抗增殖作用，这两种细胞系分别代表从低级别到高级别肿瘤的进展。在 RT112 中，EVOOE 降低了细胞活力（24 小时时的 IC50 值为 240 μg/mL），引发了非保护性自噬，这表现在自噬体的形成和 LC-3 脂质化的增加。在 J82 中，EVOOE 在 24 小时的处理后通过快速和大量的细胞凋亡强烈降低了细胞活力（IC50 值为 65.8 μg/mL），这通过 Annexin V 阳性和 caspase-3 和 -9 的激活来评估。此外，在两种膀胱癌细胞系中，EVOOE 均降低了细胞内的活性氧，但这种抗氧化作用与其抗增殖作用无关。获得的数据表明，特级初榨橄榄油中的酚类化合物混合物激活了不同的抗增殖途径。
