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基于分光光度法和液相色谱/质谱联用的脂质组学分析揭示了在冷藏期间用稳定二氧化氯处理并真空包装的海鳗脂质谱的变化。

Spectrophotometric- and LC/MS-Based Lipidomics Analyses Revealed Changes in Lipid Profiles of Pike Eel () Treated with Stable Chlorine Dioxides and Vacuum-Packed during Chilled Storage.

作者信息

Shui Shanshan, Wu Yingru, Chen Xiaonan, Li Ruixue, Yang Huicheng, Lu Baiyi, Zhang Bin

机构信息

Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.

Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, College of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.

出版信息

Foods. 2023 Jul 22;12(14):2791. doi: 10.3390/foods12142791.

Abstract

Spectrophotometric- and liquid chromatography/mass spectrometry (LC/MS)-based lipidomics analyses were performed to explore the changes of lipid profiles in pike eel () under stable chlorine dioxides (ClO) and vacuum-packed treatment during chilled storage. The peroxide value (PV) and malondialdehyde (MDA) content in ClO treated and vacuum-packaged (VP) samples were significantly reduced compared to simple-packaged (SP) samples during whole chilled storage. The LC/MS-based lipidomics analyses identified 2182 lipid species in the pike eel muscle classified into 39 subclasses, including 712 triglycerides (TGs), 310 phosphatidylcholines (PCs), 153 phosphatidylethanolamines (PEs), and 147 diglycerides (DGs), among others. Further, in comparison with fresh pike eel (FE) muscle, 354 and 164 higher and 420 and 193 lower abundant levels of differentially abundant lipids (DALs) were identified in SP samples and VP samples, respectively. Compared with the VP batch, 396 higher and 404 lower abundant levels of DALs were identified in the SP batch. Among these, PCs, PEs, TGs, and DGs were more easily oxidized/hydrolyzed, which could be used as biomarkers to distinguish FE, SP, and VP samples. This research provides a reference for controlling lipid oxidation in fatty fish.

摘要

采用基于分光光度法和液相色谱/质谱联用(LC/MS)的脂质组学分析方法,探究了在冷藏过程中,稳定二氧化氯(ClO)处理和真空包装处理对海鳗脂质谱的影响。在整个冷藏过程中,与简单包装(SP)样品相比,ClO处理和真空包装(VP)样品中的过氧化值(PV)和丙二醛(MDA)含量显著降低。基于LC/MS的脂质组学分析在海鳗肌肉中鉴定出2182种脂质,分为39个亚类,包括712种甘油三酯(TGs)、310种磷脂酰胆碱(PCs)、153种磷脂酰乙醇胺(PEs)和147种甘油二酯(DGs)等。此外,与新鲜海鳗(FE)肌肉相比,在SP样品和VP样品中分别鉴定出354种和164种丰度较高以及420种和193种丰度较低的差异丰度脂质(DALs)。与VP批次相比,在SP批次中鉴定出396种丰度较高和404种丰度较低的DALs。其中,PCs、PEs、TGs和DGs更容易被氧化/水解,可作为区分FE、SP和VP样品的生物标志物。本研究为控制高脂鱼类的脂质氧化提供了参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/663a/10379090/373dc3e3d510/foods-12-02791-g001.jpg

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