Department of Medical Microbiology and Nanobiomedical Enginnering, Medical University of Bialystok, ul. Mickiewicza 2C, 15-222 Bialystok, Poland.
Department of Microbiology and Biotechnology, University of Bialystok, ul. Ciołkowskiego 1J, 15-245 Białystok, Poland.
Genes (Basel). 2023 Jun 30;14(7):1384. doi: 10.3390/genes14071384.
is a new enteropathogen of humans and animals. The aim of the study was to assess the prevalence and pathogenicity of strains isolated in northeastern Poland using epidemiological and genomic studies. In 2015-2018, a total of 1154 fecal samples from children and adults, 497 bird droppings, 212 food samples, 92 water samples, and 500 lactose-negative strains were tested. A total of 42 strains were isolated. The PCR method was suitable for their rapid identification. In total, 33.3% of isolates were resistant to one antibiotic, and 16.7% to two. Isolates were sensitive to cefepime, imipenem, levofloxacin, gentamicin, trimethoprim/sulfamethoxazole, and did not produce ESBL β-lactamases. High genetic variability of has been demonstrated. In the PFGE method, 90.5% of the strains had distinct pulsotypes. In MLST typing, 85.7% of strains were assigned distinct sequence types (STs), of which 64% were novel ST types. Cytolethal distending toxin (CDT) and Paa toxin genes were found in 100% of isolates. Genes encoding toxins, IbeA, CdtB type 2, Tsh and Shiga (Stx2f), were found in 26.2%, 9.7%, 1.7%, and 0.4% of isolates, respectively. The chromosome size of the tested strains ranged from 4,573,338 to 5,141,010 bp (average 4,784,003 bp), and at least one plasmid was present in all strains. The study contributes to a more accurate assessment of the genetic diversity of and the potential threat it poses to public health.
是一种新的人类和动物肠道病原体。本研究的目的是通过流行病学和基因组研究评估在波兰东北部分离的 菌株的流行率和致病性。2015-2018 年，共检测了 1154 份儿童和成人粪便样本、497 份鸟粪、212 份食品样本、92 份水样和 500 份乳糖阴性 菌株。共分离出 42 株 。PCR 方法适合其快速鉴定。共有 33.3%的 分离株对一种抗生素耐药，16.7%的分离株对两种抗生素耐药。分离株对头孢吡肟、亚胺培南、左氧氟沙星、庆大霉素、复方磺胺甲噁唑敏感，不产生 ESBL 型β-内酰胺酶。已证明 的遗传变异性很高。在 PFGE 方法中，90.5%的菌株具有明显的脉冲型。在 MLST 分型中，85.7%的菌株被分配到不同的序列类型（ST），其中 64%为新型 ST 类型。100%的 分离株中发现细胞致死膨胀毒素（CDT）和 Paa 毒素基因。编码毒素、IbeA、CdtB 型 2、Tsh 和志贺（Stx2f）的基因分别在 26.2%、9.7%、1.7%和 0.4%的 分离株中发现。检测菌株的染色体大小范围为 4573338-5141010bp（平均 4784003bp），所有菌株中至少存在一个质粒。该研究有助于更准确地评估 的遗传多样性及其对公共健康的潜在威胁。
