Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
College of Science, Northeastern University, Boston, MA 02115, USA.
Genes (Basel). 2023 Jul 5;14(7):1400. doi: 10.3390/genes14071400.
is an important winter-growing cover crop. It is widely utilized, not only as a cover crop for its benefits in fertilizing the soil but also as a landscape ground cover plant. Anthocyanins are involved in the pigmentation of plants in leaves and flowers, which is a crucial characteristic trait for The formation of anthocyanins depends significantly on the enzyme chalcone isomerase (CHI). However, research on the gene of remains unexplored. The rapid amplification of cDNA ends (RACE) approach was used in this research to clone the sequence from ). The expression profiles of the gene in multiple tissues of were subsequently examined by qRT-PCR (Quantitative Real-Time PCR). Furthermore, the function of the was identified by the performance of ectopic expression in (). The outcomes revealed that the full-length cDNA of the gene (GeneBank: OQ870547) measured 972 bp in length and included an open reading frame of 660 bp. The encoded protein contains 219 amino acids with a molecular weight of 24.14 kDa and a theoretical isoelectric point of 5.11. In addition, the remarkable similarity between the AsiCHI protein and the CHI proteins of other species was demonstrated by the sequence alignment and phylogenetic analysis. Moreover, the highest expression level of was observed in leaves and showed a positive correlation with anthocyanin content. The functional analysis further revealed that the overexpression of enhanced the anthocyanidin accumulation in the transgenic lines. This study provided a better understanding of and elucidated its role in anthocyanin production.
是一种重要的冬季生长的覆盖作物。它被广泛应用，不仅作为一种为土壤施肥的覆盖作物，还作为一种景观地面覆盖植物。花色素苷参与植物叶片和花朵的色素形成，这是一个重要的特征性状。花色素苷的形成很大程度上依赖于查尔酮异构酶（CHI）。然而，关于 的 基因的研究尚未探索。本研究采用快速扩增 cDNA 末端（RACE）方法从 克隆 序列。随后通过 qRT-PCR（定量实时 PCR）检测 基因在 多个组织中的表达谱。此外，通过在 （）中异位表达鉴定了 的功能。结果表明， 基因的全长 cDNA（基因库：OQ870547）长 972bp，包含 660bp 的开放阅读框。编码的蛋白含有 219 个氨基酸，分子量为 24.14kDa，理论等电点为 5.11。此外，序列比对和系统发育分析表明，AsiCHI 蛋白与其他 物种的 CHI 蛋白具有显著的相似性。此外，在叶片中观察到 的表达水平最高，并与花色素苷含量呈正相关。功能分析进一步表明， 的过表达增强了转基因系中花色素苷的积累。本研究加深了对 的理解，并阐明了其在花青素合成中的作用。
