Reference Centre for Lactobacilli (CERELA-CONICET), Tucumán 4000, Argentina.
Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.
Int J Mol Sci. 2023 Jul 11;24(14):11309. doi: 10.3390/ijms241411309.
, the type species of the genus , is widely recognized as the primary starter culture in the dairy industry due to its proteolytic activity, which enables it to growth in milk. In this study, a comprehensive genomic analysis of the proteolytic system was conducted on strains. The analysis included 27 genomes of , with a specific focus on the key enzyme involved in this system, the cell envelope-associated proteinase (CEP). The amino acid sequences, as well as the protein-structure prediction of the CEPs, were compared. Additionally, syntenic analysis of the genomic locus related to the CEPs revealed high conservation in subsp. strains, while subsp. strains exhibited greater variability, including the presence of insertion sequences, deletions, and rearrangements. Finally, the CEP promoter region and putative regulatory elements responsible for controlling the expression of the proteolytic system in lactobacilli were investigated. Our genomic analysis and characterization of the CEPs contribute to our understanding of proteolytic activity and the potential applications of these lactic acid bacteria in the dairy industry. Further research in this area will expand our knowledge and potential practical uses of these findings.
作为 属的模式种，因其具有蛋白水解活性而被广泛认为是乳制品工业中的主要起始培养物，这种活性使其能够在牛奶中生长。在这项研究中，对 菌株的蛋白水解系统进行了全面的基因组分析。该分析包括 27 个 基因组，特别关注该系统中的关键酶，即细胞周质相关蛋白酶（CEP）。比较了 CEPs 的氨基酸序列和蛋白质结构预测。此外，与 CEPs 相关的基因组位点的同线性分析表明， 亚种菌株具有高度的保守性，而 亚种菌株则表现出更大的变异性，包括插入序列、缺失和重排的存在。最后，研究了 CEP 启动子区域和可能负责调控乳杆菌蛋白水解系统表达的调节元件。我们的基因组分析和对 CEPs 的 特性研究有助于我们理解蛋白水解活性以及这些乳酸菌在乳制品工业中的潜在应用。该领域的进一步研究将扩展我们对这些发现的知识和潜在实际用途。
