Stinson Lisa F, Berman Yey, Li Shaofu, Keelan Jeffrey A, Dickinson Jan E, Doherty Dorota A, Newnham John P, Payne Matthew S
School of Molecular Sciences, The University of Western Australia, Perth, WA 6009, Australia.
Division of Obstetrics and Gynaecology, The University of Western Australia, Perth, WA 6008, Australia.
Microorganisms. 2023 Jun 29;11(7):1698. doi: 10.3390/microorganisms11071698.
A well-established association exists between intrauterine bacteria and preterm birth. This study aimed to explore this further through documenting bacterial and cytokine profiles in Australian mid-gestation amniotic fluid samples from preterm and term births. Samples were collected during amniocenteses. DNA was extracted and the full-length 16S rRNA gene was amplified and sequenced. Levels of the cytokines IL-1β, IL-6, IL-10, TNF-α and MCP-1 were determined using the Milliplex MAGPIX system. Bacterial DNA profiles were low in diversity and richness, with no significant differences observed between term and preterm samples. No differences in the relative abundance of individual OTUs between samples were identified. IL-1β and TNF-α levels were significantly higher in samples containing reads mapping to sp.; however, this result should be interpreted with caution as similar reads were also identified in extraction controls. IL-6 levels were significantly increased in samples with reads that mapped to sp., whilst TNF-α levels were elevated in fluid samples from pregnancies that subsequently delivered preterm. Bacterial DNA unlikely to have originated from extraction controls was identified in 20/31 (64.5%) mid-gestation amniotic fluid samples. Bacterial DNA profiles, however, were not predictive of preterm birth, and although cytokine levels were elevated in the presence of certain genera, the biological relevance of this remains unknown.
子宫内细菌与早产之间存在着一种已被充分证实的关联。本研究旨在通过记录澳大利亚早产和足月产的妊娠中期羊水样本中的细菌和细胞因子谱，进一步探究这一关联。样本在羊膜穿刺术期间采集。提取DNA并对全长16S rRNA基因进行扩增和测序。使用Milliplex MAGPIX系统测定细胞因子IL-1β、IL-6、IL-10、TNF-α和MCP-1的水平。细菌DNA谱的多样性和丰富度较低，足月和早产样本之间未观察到显著差异。未发现样本之间单个OTU相对丰度的差异。在含有与sp.匹配读数的样本中，IL-1β和TNF-α水平显著更高；然而，由于在提取对照中也鉴定出了类似的读数，因此对这一结果的解释应谨慎。在与sp.匹配读数的样本中，IL-6水平显著升高，而在随后早产的妊娠的羊水样本中，TNF-α水平升高。在31份妊娠中期羊水样本中的20份（64.5%）中鉴定出了不太可能源自提取对照的细菌DNA。然而，细菌DNA谱并不能预测早产，尽管在某些菌属存在的情况下细胞因子水平升高，但其生物学相关性仍然未知。
