Grishkan Isabella, Kidron Giora J, Rodriguez-Berbel Natalia, Miralles Isabel, Ortega Raúl
Institute of Evolution, University of Haifa, 199 Aba Khoushy Ave, Mount Carmel, Haifa 3498838, Israel.
Institute of Earth Sciences, The Hebrew University of Jerusalem, Givat Ram Campus, Jerusalem 91904, Israel.
Microorganisms. 2023 Jul 5;11(7):1761. doi: 10.3390/microorganisms11071761.
We examined fungal communities in soil profiles of 0-10 cm depth along the altitudinal gradient of 250-530-990 m.a.s.l. at the Central Negev Desert, Israel, which benefit from similar annual precipitation (95 mm). In the soil samples collected in the summer of 2020, a mycobiota accounting for 169 species was revealed by both culture-dependent and culture-independent (DNA-based) methodologies. The impact of soil depth on the variations in fungal communities was stronger than the impact of altitude. Both methodologies displayed a similar tendency in the composition of fungal communities: the prevalence of melanin-containing species with many-celled large spores (mainly spp.) in the uppermost layers and the depth-wise increase in the proportion of light-colored species producing a high amount of small one-celled spores. The culturable and the DNA-based fungal communities had only 13 species in common. The differences were attributed to the pros and cons of each method. Nevertheless, despite the drawbacks, the employment of both methodologies has an advantage in providing a more comprehensive picture of fungal diversity in soils.
我们研究了以色列内盖夫沙漠中部海拔250 - 530 - 990米处深度为0 - 10厘米的土壤剖面中的真菌群落，这些地区年降水量相似（95毫米）。在2020年夏季采集的土壤样本中，通过依赖培养和不依赖培养（基于DNA）的方法揭示了一个由169种真菌组成的菌群。土壤深度对真菌群落变化的影响比海拔的影响更强。两种方法在真菌群落组成上显示出相似的趋势：在最上层，含黑色素且具有多细胞大孢子的物种（主要是 属）占优势，而产生大量单细胞小孢子的浅色物种比例则随深度增加。可培养的真菌群落和基于DNA的真菌群落仅有13种相同。差异归因于每种方法的优缺点。然而，尽管存在缺点，但同时使用这两种方法在更全面地了解土壤真菌多样性方面具有优势。
