采用亚磷酰胺化学法合成骨架修饰的吗啉代寡核苷酸。

Nucleic Acid Solutions Division, Agilent Technologies, Boulder, CO 80301, USA.

Department of Biochemistry, University of Colorado, Boulder, CO 80303, USA.

Molecules. 2023 Jul 13;28(14):5380. doi: 10.3390/molecules28145380.

Phosphorodiamidate morpholinos (PMOs) are known as premier gene knockdown tools in developmental biology. PMOs are usually 25 nucleo-base-long morpholino subunits with a neutral phosphorodiamidate linkage. PMOs work via a steric blocking mechanism and are stable towards nucleases' inside cells. PMOs are usually synthesized using phosphoramidate P(V) chemistry. In this review, we will discuss the synthesis of PMOs, phosphoroamidate morpholinos (MO), and thiophosphoramidate morpholinos (TMO).

磷酰二胺吗啉代寡聚物（PMO）在发育生物学中被称为主要的基因敲低工具。PMO 通常是 25 个核苷酸长的吗啉代亚基，具有中性的磷酰二胺键。PMO 通过空间位阻阻断机制发挥作用，并且在细胞内对核酸酶稳定。PMO 通常使用磷酰胺 P(V)化学合成。在这篇综述中，我们将讨论 PMO、磷酰氨酯吗啉代寡聚物（MO）和硫代磷酰氨酯吗啉代寡聚物（TMO）的合成。