Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, Poland.
Molecules. 2023 Jul 15;28(14):5422. doi: 10.3390/molecules28145422.
is a little-understood plant with unexplored biological potential, and the papers regarding its chemical composition are scarce. In our study, for the first time, the phytochemical profile of the plant, focusing on polar metabolites, was established using modern chromatographic techniques including LC-HRMS-QTOF-CAD, UHPLC-PDA-MS. Phytochemical analysis revealed that the species is a rich source of polyphenolic components, with the most abundant being chlorogenic acid and C-glycosides of luteolin, including carlinoside, orientin, isoorientin, and C-glycosides of apigenin, schaftoside, isoschaftoside, and vitexin. Furthermore, we assessed the impact of the polyphenolic-rich fraction of extracts on human skin fibroblasts using the MTT and NR assays. It was found that the extract was non-toxic and exhibited potent antioxidant activity in the cells subjected to induced oxidative stress. Additionally, it effectively protected the cells against HO-induced cytotoxicity. Our study contributes to the general trend of searching for new phytotherapeutics with potential applications in pharmacy and medicine. The results indicate that further exploration of species is worthwhile, as they can serve as valuable plant material for cosmetic use.
是一种人们了解甚少的植物，具有尚未开发的生物潜力，关于其化学成分的文献也很少。在我们的研究中，首次使用现代色谱技术，包括 LC-HRMS-QTOF-CAD、UHPLC-PDA-MS，建立了该植物的植物化学成分分析，重点是极性代谢物。植物化学分析表明，该物种是多酚类成分的丰富来源，其中最丰富的是绿原酸和木樨草素的 C-糖苷，包括梓醇苷、橙皮苷、异橙皮苷和芹菜素、山柰酚、异山柰酚和牡荆素的 C-糖苷。此外，我们使用 MTT 和 NR 测定法评估了多酚丰富提取物对人皮肤成纤维细胞的影响。结果发现，提取物无毒，在经受诱导氧化应激的细胞中表现出强大的抗氧化活性。此外，它还能有效保护细胞免受 HO 诱导的细胞毒性。我们的研究有助于寻找具有在药学和医学中应用潜力的新型植物疗法的总体趋势。研究结果表明，进一步探索该物种是值得的，因为它们可以作为有价值的植物材料用于化妆品。
