Yang Jun, Li Qingyun, Wang Lu, Xie Hui
Clinical Medical College, Chengdu University of Traditional Chinese Medicine, Chengdu 610036, China.
Clinical Medical College, Chengdu University of Traditional Chinese Medicine, Chengdu 610036, China. *Corresponding author, E-mail:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2023 Aug;39(8):680-685.
Objective To investigate the role of microRNA-18a (miR-18a) in the pathogenesis of allergic rhinitis in mice. Methods Twenty-two BALB/c mice were randomly divided into a blank group, a model group and a miR-18a group. Mice in the model group and the miR-18a group were injected intraperitoneally with obumin (OVA) suspension to prepare allergic rhinitis models, and mice in the miR-18a group were simultaneously given lentiviral vector plasmid for overexpression of miR-18a. Allergy symptoms were evaluated by the behavioral score and HE staining. The plasma levels of interleukin-1β (IL-1β), IL-6 and tumor necrosis factor α (TNF-α) were measured by ELISA. The distribution of CD45 cells in nasal mucosa was measured by immunofluorescence histochemistry, and CD45 cells in nasal lavage fluid were measured by flow cytometry. The mRNA expression levels of IL-1β, IL-6 and TNF-α in nasal mucosa tissues were measured by fluorescence quantitative PCR, and the protein expressions of Toll like receptor 4 (TLR4), nuclear factor κB p65 (NF-κB p65), inhibitor of NF-κB α (IκBα) and phosphorylated IκBα (p-IκBα) in nasal mucosa were measured by Western blot analysis. Results Compared with the blank group, the plasma levels of IL-1β, IL-6, and TNF-α in the model group increased significantly. The number of CD45 cells in both nasal mucosa tissue and nasal irrigation fluid increased, and the mRNA levels of IL-1β, IL-6 and TNF-α and the protein expression levels of TLR4, NF-κB p65 and p-IκBα in nasal mucosa increased. Compared with the model group, the plasma levels of IL-1β, IL-6 and TNF-α in the miR-18a group decreased significantly. The number of CD45 cells in both nasal mucosa tissue and nasal lavage fluid decreased, and the mRNA levels of IL-1β, IL-6 and TNF-α and the exprotein expression levels of TLR4, NF-κB p65 and p-IκBα in nasal mucosa decreased. Conclusion miR-18a can inhibit the occurrence and development of allergic rhinitis, and its molecular mechanism is related to the inhibition of TLR4/NF-κB pathway activation.
目的 探讨微小RNA-18a（miR-18a）在小鼠变应性鼻炎发病机制中的作用。方法 将22只BALB/c小鼠随机分为空白组、模型组和miR-18a组。模型组和miR-18a组小鼠腹腔注射卵清蛋白（OVA）混悬液制备变应性鼻炎模型，miR-18a组小鼠同时给予慢病毒载体质粒以过表达miR-18a。通过行为学评分和HE染色评估过敏症状。采用酶联免疫吸附测定（ELISA）法检测血浆白细胞介素-1β（IL-1β）、IL-6和肿瘤坏死因子α（TNF-α）水平。采用免疫荧光组织化学法检测鼻黏膜中CD45细胞的分布，采用流式细胞术检测鼻灌洗液中CD45细胞。采用荧光定量聚合酶链反应（PCR）检测鼻黏膜组织中IL-1β、IL-6和TNF-α的mRNA表达水平，采用蛋白质免疫印迹法检测鼻黏膜中Toll样受体4（TLR4）、核因子κB p65（NF-κB p65）、NF-κBα抑制蛋白（IκBα）和磷酸化IκBα（p-IκBα）的蛋白表达。结果 与空白组比较，模型组血浆IL-1β、IL-6和TNF-α水平显著升高。鼻黏膜组织和鼻灌洗液中CD45细胞数量增加，鼻黏膜中IL-1β、IL-6和TNF-α的mRNA水平以及TLR4、NF-κB p65和p-IκBα的蛋白表达水平升高。与模型组比较，miR-18a组血浆IL-1β、IL-6和TNF-α水平显著降低。鼻黏膜组织和鼻灌洗液中CD45细胞数量减少，鼻黏膜中IL-1β、IL-6和TNF-α的mRNA水平以及TLR4、NF-κB p65和p-IκBα的蛋白表达水平降低。结论 miR-18a可抑制变应性鼻炎的发生发展，其分子机制与抑制TLR4/NF-κB信号通路激活有关
