Applied Genomics Section, Bhabha Atomic Research Centre, Mumbai, India.
Homi Bhabha National Institute, Mumbai, India.
J Med Virol. 2023 Aug;95(8):e28974. doi: 10.1002/jmv.28974.
Mpox virus, a member of genus Orthopoxvirus, causes rash and flu-like symptoms in humans. In the recent global outbreak, it was reported from several geographical areas that have not historically reported mpox. Point of care, sensitive and specific mpox diagnostic assays are critical in checking the spread of the disease. We have developed a clustered regularly interspaced short palindromic repeats associated Cas12a nuclease-based assay for detecting mpox virus. Mpox specific conserved sequences were identified in polA (E9L) gene which differ by a single nucleotide polymorphism (SNP) from all the viruses present in the genus Orthopoxvirus. This SNP was exploited in our assay to specifically distinguish mpox virus from other related orthopox viruses with a limit of detection of 1 copy/μl in 30 min. The assay exhibits a sensitive and specific detection of mpox virus which can prove to be of practical value for its surveillance in areas infected with multiple orthopox viruses, especially in hotspots of mpox virus infections.
猴痘病毒是正痘病毒属的一员，会导致人类出现皮疹和流感样症状。在最近的全球疫情中，报告了几个历史上没有报告过猴痘的地理区域。在检查疾病的传播时，即时、敏感和特异性的猴痘诊断检测至关重要。我们开发了一种基于聚合酶链反应（PCR）的检测方法，用于检测猴痘病毒。在 polA (E9L) 基因中鉴定出猴痘特异性保守序列，该序列与正痘病毒属中的所有病毒存在单核苷酸多态性（SNP）差异。我们的检测方法利用了这一 SNP，可特异性区分猴痘病毒与其他相关的正痘病毒，在 30 分钟内的检测限为 1 拷贝/μl。该检测方法对猴痘病毒具有灵敏和特异性的检测能力，这对于在感染多种正痘病毒的地区进行监测，特别是在猴痘病毒感染的热点地区，可能具有实际价值。
