State Key Laboratory of Medicinal Chemical Biology, Department of Biochemistry and Molecular Biology, College of Life Sciences, Nankai University, Tianjin, China.
Plant Signal Behav. 2023 Dec 31;18(1):2241165. doi: 10.1080/15592324.2023.2241165.
The plant-specific stress response protein NRP (asparagine-rich protein) is characterized by an asparagine-rich domain at its N-terminus and a conserved development and cell death (DCD) domain at its C-terminus. Previous transcriptional studies and phenotypic analyses have demonstrated the involvement of NRP in response to severe stress conditions, such as high salt and ER Endoplasmic reticulum-stress. We have recently identified distinct roles for NRP in biotic- and abiotic-stress signaling pathways, in which NRP interacts with different signaling proteins to change their subcellular localizations and stability. Here, to further explore the function of NRP, a transcriptome analysis was carried out on knock-out lines at different life stages or under different growing conditions. The most significant changes in the transcriptome at both stages and conditions turned out to be the induction of the synthesis of secondary metabolites (SMs). Such an observation implicates that NRP is a general stress-responsive protein involved in various challenges faced by plants during their life cycle, which might involve a broad alteration in the distribution of SMs.
植物特异性应激反应蛋白 NRP（富含天冬酰胺的蛋白）的特点是其 N 端有一个富含天冬酰胺的结构域，C 端有一个保守的发育和细胞死亡（DCD）结构域。先前的转录研究和表型分析表明，NRP 参与了对严重应激条件的反应，如高盐和内质网应激。我们最近发现 NRP 在生物和非生物应激信号通路中具有不同的作用，其中 NRP 与不同的信号蛋白相互作用，改变它们的亚细胞定位和稳定性。在这里，为了进一步探索 NRP 的功能，我们在不同的生命阶段或不同的生长条件下对敲除系进行了转录组分析。在这两个阶段和条件下，转录组最显著的变化是诱导次生代谢物（SMs）的合成。这一观察结果表明，NRP 是一种普遍的应激反应蛋白，参与植物在其生命周期中所面临的各种挑战，这可能涉及到 SMs 分布的广泛改变。
