College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, Gansu 730070, PR China.
Animal Science and Veterinary Institute of Gannan Prefecture, Gannan Tibetan Autonomous Prefecture, Gansu 747000, PR China.
Meat Sci. 2023 Oct;204:109264. doi: 10.1016/j.meatsci.2023.109264. Epub 2023 Jun 20.
The present study aimed to characterize the influence of hypoxia-inducible factor-1α on heat shock protein 27 and cytochrome c translocation, yak meat microstructure destruction, and endogenous enzymes activities, refining the understanding of the tenderization process after slaughter. Postmortem yak longissimus thoracis et lumborum muscles were incubated with 0.9% saline or hypoxia-inducible factor-1α stabilizer dimethyloxaloylglycine at 4 °C for 6, 12, 24, 72, and 120 h. Results showed that hypoxia-inducible factor-1α activation promoted heat shock protein 27 migration and cytochrome c release, facilitating (P < 0.05) caspase-3 activity by mediating the heat shock protein 27/caspase-3 interaction but did not exert (P > 0.05) significant effects on the calpain-1 activity. Additionally, hypoxia-inducible factor-1α activation contributed to the mitochondrial apoptosis cascade, leading to a higher (P < 0.01) apoptosis rate. Therefore, these observations indicate that hypoxia-inducible factor-1α affects caspase-3 activity and tenderness of postmortem muscle through distinct regulatory mechanisms, possibly, in part, with heat shock protein 27 and cytochrome c mediation.
本研究旨在探讨缺氧诱导因子-1α对热休克蛋白 27 和细胞色素 c 易位、牦牛肌肉微观结构破坏以及内源性酶活性的影响，以深入了解宰后嫩化过程。将牦牛背最长肌在 4°C 下用 0.9%生理盐水或缺氧诱导因子-1α稳定剂二甲草酰甘氨酸孵育 6、12、24、72 和 120 h。结果表明，缺氧诱导因子-1α的激活促进了热休克蛋白 27 的迁移和细胞色素 c 的释放，通过介导热休克蛋白 27/半胱天冬酶-3 的相互作用，显著促进了（P<0.05）半胱天冬酶-3 的活性，但对钙蛋白酶-1的活性没有显著影响（P>0.05）。此外，缺氧诱导因子-1α的激活导致线粒体凋亡级联反应，从而导致更高的（P<0.01）凋亡率。因此，这些观察结果表明，缺氧诱导因子-1α可能通过不同的调节机制影响死后肌肉的半胱天冬酶-3 活性和嫩度，可能部分通过热休克蛋白 27 和细胞色素 c 介导。
