Department of Solar Materials - Microscale Analysis and Engineering, Helmholtz-Centre for Environmental Research - UFZ Leipzig, Leizpig, Germany.
Department of Solar Materials - Microscale Analysis and Engineering, Helmholtz-Centre for Environmental Research - UFZ Leipzig, Leizpig, Germany.
Curr Opin Biotechnol. 2023 Oct;83:102977. doi: 10.1016/j.copbio.2023.102977. Epub 2023 Jul 27.
Single-cell analysis uncovers phenotypic differences between cells in a population and dissects their individual physiological states and differences on all omics levels from genome to phenome. Spectrometric observation allows label-free analysis of the metabolome and proteome of individual cells, but is still mainly limited to the analysis of mammalian single cells. Recent progress in mass spectrometry approaches now enables the analysis of microbial single cells - mainly by miniaturizing cell handling, incubation, and improving chip-coupling concepts for analyte ionization by interfacing microfluidic chips and mass spectrometers. This review aims at distilling the enabling principles behind microbial single-cell mass spectrometry and puts them into perspective for the future of the field.
单细胞分析揭示了群体中细胞之间的表型差异，并剖析了它们在基因组到表型各个组学层面上的个体生理状态和差异。光谱观测允许对单个细胞的代谢组和蛋白质组进行无标记分析，但仍然主要局限于对哺乳动物单细胞的分析。目前，质谱分析方法的最新进展使得微生物单细胞的分析成为可能，主要是通过微型化细胞处理、孵育以及通过将微流控芯片与质谱仪相连接来改善分析物离子化的芯片耦合概念。这篇综述旨在提炼微生物单细胞质谱背后的使能原理，并为该领域的未来提供展望。
