植物乳杆菌表面展示的柔嫩艾美耳球虫原肌球蛋白抗原与 FliC 鞭毛蛋白可诱导鸡对球虫病的保护。
Lactobacillus plantarum surface-displayed Eimeria tenella profilin antigens with FliC flagellin elicit protection against coccidiosis in chickens.
机构信息
College of Veterinary Medicine, Jilin Agricultural University, Changchun, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China; Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun, China.
College of Veterinary Medicine, Jilin Agricultural University, Changchun, China; Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China; Engineering Research Center of Microecological Vaccines (Drugs) for Major Animal Diseases, Ministry of Education, Jilin Agricultural University, Changchun, China.
出版信息
Poult Sci. 2023 Oct;102(10):102945. doi: 10.1016/j.psj.2023.102945. Epub 2023 Jul 18.
Coccidiosis is a parasitic disease in the intestine caused by the genus Eimeria that poses a substantial economic threat to the broiler breeding industry. The misuse of chemoprophylaxis and live oocyst vaccines has a negative impact on chicken reproductivity. Therefore, there is a pressing need to develop safe, convenient, and effective vaccines. Lactic acid bacteria can be used as a means to deliver mucosal vaccines against intestinal pathogens, which is a promising strategy. In this study, a recombinant Lactobacillus plantarum (L. plantarum) with surface-expressed antigens constructed from the fusion of Eimeria tenella (E. tenella) antigen profilin and the Salmonella enterica serovar Typhimurium flagellin protein FliC was created. After oral immunization with the recombinant L. plantarum, T-cell differentiation was analyzed by flow cytometry, and specific antibody levels were determined via indirect ELISA. Oocyst shedding, body weight, and cecum lesions were assessed as measures of protective immunity after challenge with E. tenella. The results of this study demonstrate the effectiveness of recombinant L. plantarum as an immunization agent for chickens. Specific IgA titers in the intestine and specific IgG antibody titers in the serum were significantly higher in chickens immunized with recombinant L. plantarum (P < 0.001). Additionally, the levels of IL-2 (P < 0.05) and IFN-γ (P < 0.01) in the serum were markedly increased. Recombinant L. plantarum induced T-cell differentiation, resulting in a higher proportion of CD4 and CD8 T cells in splenocytes (P < 0.001). Fecal oocyst shedding in the immunized group was significantly reduced (P < 0.001). Additionally, recombinant L. plantarum significantly relieved pathological damage in the cecum, as evidenced by lesion scores (P < 0.01) and histopathological cecum sections. In conclusion, the present study provides evidence to support the possibility of using L. plantarum as a promising carrier for the delivery of protective antigens to effectively protect chickens against coccidiosis.
球虫病是一种由艾美耳属寄生虫引起的肠道寄生虫病，对肉鸡养殖业构成了重大的经济威胁。化学预防和活卵囊疫苗的滥用对鸡的繁殖性能产生负面影响。因此，迫切需要开发安全、方便、有效的疫苗。乳酸菌可作为传递针对肠道病原体的粘膜疫苗的手段，这是一种很有前途的策略。在这项研究中，构建了一种表面表达抗原的重组植物乳杆菌（L. plantarum），该抗原由柔嫩艾美耳球虫（E. tenella）抗原微丝蛋白和沙门氏菌血清型鼠伤寒杆菌鞭毛蛋白 FliC 的融合而成。通过口服免疫重组 L. plantarum，通过流式细胞术分析 T 细胞分化，并通过间接 ELISA 测定特异性抗体水平。通过用柔嫩艾美耳球虫攻毒来评估卵囊脱落、体重和盲肠病变作为保护免疫的衡量标准。这项研究的结果表明，重组 L. plantarum 作为鸡的免疫制剂是有效的。用重组 L. plantarum 免疫的鸡，肠道中的特异性 IgA 滴度和血清中的特异性 IgG 抗体滴度均显著升高（P < 0.001）。此外，血清中 IL-2（P < 0.05）和 IFN-γ（P < 0.01）的水平明显升高。重组 L. plantarum 诱导 T 细胞分化，导致脾细胞中 CD4 和 CD8 T 细胞的比例更高（P < 0.001）。免疫组粪便卵囊脱落明显减少（P < 0.001）。此外，重组 L. plantarum 显著缓解了盲肠的病理损伤，表现为病变评分（P < 0.01）和盲肠组织切片的组织病理学变化。总之，本研究提供了证据支持将植物乳杆菌用作保护性抗原传递的有前途载体的可能性，以有效保护鸡免受球虫病的侵害。
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