Török B, Röth E, Matkovics B
Acta Physiol Hung. 1986;68(1):25-31.
Dog experiments were performed to establish the lipid peroxidation of heart tissue (measured by formation of malone-dialdehyde--MDA) and the natural scavenger action (measured by determination of superoxide dismutase--SOD and of reduced glutathione--GSH). Experimental groups were: control dogs having intact heart, dogs ventilated with hypoxic gas (N2O and O2 at a ratio of 10:1) for 1, 2 and 3 hours and dogs having acute coronary ligature for 1, 2, 3 and 24 hours. Acute hypoxia caused a gradual increase of MDA concentration, a moderate increase of the GSH level and a sharp decrease in SOD activity. In ischaemic heart tissue, these changes were very distinctive. High MDA values were found after 3 hours. GSH level and SOD activity decreased continually. Increased MDA formation indicates breakdown of polyunsaturated fatty acids (PUFA) in the membranes, decreased GSH and SOD levels indicate impairment of the natural scavenging, clearly outlining the extent of disintegration of the membrane structure and function due to the effect of toxic free oxygen radicals.
进行了犬类实验，以确定心脏组织的脂质过氧化作用（通过丙二醛——MDA的形成来衡量）以及天然清除剂作用（通过超氧化物歧化酶——SOD和还原型谷胱甘肽——GSH的测定来衡量）。实验组包括：心脏完好的对照犬、用低氧气体（N₂O和O₂比例为10:1）通气1、2和3小时的犬，以及急性冠状动脉结扎1、2、3和24小时的犬。急性缺氧导致MDA浓度逐渐升高、GSH水平适度升高以及SOD活性急剧下降。在缺血性心脏组织中，这些变化非常明显。3小时后发现MDA值较高。GSH水平和SOD活性持续下降。MDA形成增加表明膜中多不饱和脂肪酸（PUFA）分解，GSH和SOD水平降低表明天然清除功能受损，清楚地勾勒出由于有毒的游离氧自由基的作用，膜结构和功能的解体程度。
