铜质接触表面对 MS2 噬菌体和甲型肝炎病毒的抗病毒活性。
Antiviral activity of copper contact surfaces against MS2 coliphage and hepatitis a virus.
机构信息
Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, 6502 S. Archer Rd, Bedford Park, IL 60501, USA.
出版信息
J Appl Microbiol. 2023 Aug 1;134(8). doi: 10.1093/jambio/lxad160.
AIMS
Viral diseases can be indirectly transmitted by contaminated non-food contact surfaces to final food products by cross-contamination. The interaction of metal surfaces and viruses, MS2 coliphage and hepatitis A virus (HAV), was investigated for strategy development in decreasing this transmission risk.
METHODS AND RESULTS
MS2 deposited onto stainless-steel surface was stable but inactivated at 0.95 log10 PFU min-1 on 99.9% copper surfaces. Greater copper-inactivation of MS2 was observed in (a) simple media (phosphate buffered saline, PBS) than protein-rich media (beef extract buffer), and (b) acidic than pH ≥ 6.8 environments. Among food matrices (strawberry juices and beef broth), the greatest MS2 inactivation by copper occurred in filtered strawberry juice at pH 3.5. At a reduction of 0.17 log10 PFU min-1, HAV survived longer than MS2 on copper by FRhK-4 cell infectivity assay.
CONCLUSIONS
The inactivation of virus on copper surfaces was greater in acidic viral surrounding environments and in simple PBS medium. In the same 99% PBS medium, MS2 may not be an appropriate surrogate for HAV when assessing viral inactivation on copper surfaces.
目的
病毒可通过污染的非食品接触面间接传播至最终食品，造成交叉污染。本研究旨在通过调查金属表面与病毒（MS2 噬菌体和甲型肝炎病毒）之间的相互作用，为降低这种传播风险制定策略。
方法和结果
在 99.9%铜表面，MS2 可在不锈钢表面稳定存在，但以 0.95 log10 PFU min-1 的速度失活。在（a）简单介质（磷酸盐缓冲盐水，PBS）中，而非富含蛋白质的介质（牛肉提取物缓冲液）中，MS2 在铜表面的失活速度更快；（b）在酸性条件（pH 值＜6.8）下，MS2 在铜表面的失活速度更快。在草莓汁和牛肉汤等食品基质中，在 pH 值为 3.5 的过滤草莓汁中，铜对 MS2 的灭活效果最佳。采用 FRhK-4 细胞感染试验，与 MS2 相比，HAV 在铜表面的存活时间更长，减少 0.17 log10 PFU min-1。
结论
在酸性病毒环境和简单的 PBS 介质中，病毒在铜表面的失活速度更快。在相同的 99% PBS 介质中，当评估铜表面病毒失活时，MS2 可能不适合作为 HAV 的替代物。
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