College of Life Science, Shaanxi Normal University, Xi'an 710119, People's Republic of China.
Basic Experimental Teaching Center, Shaanxi Normal University, Xi'an 710119, People's Republic of China.
Comp Biochem Physiol Part D Genomics Proteomics. 2023 Sep;47:101113. doi: 10.1016/j.cbd.2023.101113. Epub 2023 Jul 24.
Anurans have been excellent organisms for studying amphibian metamorphosis. Tail resorption is a remarkable event that occurs during amphibian metamorphosis. Although tail resorption has been previously studied in other anurans like Xenopus laevis and Rana chensinensis, there is no report on Bufo gargarizans. This paper thus explored the mechanism of tail resorption during metamorphosis in Bufo gargarizans tadpoles through some biological research methods. Histological results showed that the tail tissues of tadpoles gradually degraded as metamorphosis progressed. RNA sequencing analysis was performed to examine the expression level and functional enrichment of differentially expressed genes in the tail. In addition, we analyzed the mRNA expression levels of genes related to tail resorption by quantitative real-time polymerase chain reaction. We also speculated on three pathways that participate in the regulation of tail resorption based on the above results. The present study might provide a theoretical basis and novel insights for further research of complex molecular mechanisms of tail resorption in amphibians.
蛙类一直是研究两栖动物变态发育的优秀生物。尾部吸收是两栖动物变态发育过程中发生的一个显著事件。虽然尾部吸收已经在其他蛙类如非洲爪蟾和中华蟾蜍中进行了研究，但在泽蛙中尚无报道。因此，本文通过一些生物学研究方法，探讨了泽蛙蝌蚪变态过程中尾部吸收的机制。组织学结果表明，随着变态的进行，蝌蚪的尾部组织逐渐降解。通过 RNA 测序分析，检测了尾巴中差异表达基因的表达水平和功能富集情况。此外，通过定量实时聚合酶链反应分析了与尾部吸收相关基因的 mRNA 表达水平。我们还根据上述结果推测了参与尾部吸收调控的三个途径。本研究可能为进一步研究两栖动物尾部吸收的复杂分子机制提供理论基础和新的见解。
