早产儿肠道微生物网络的保守共变及其对生命早期 5 年生长的影响：一项前瞻性队列研究。
Conserved Covarying Gut Microbial Network in Preterm Infants and Childhood Growth During the First 5 Years of Life: A Prospective Cohort Study.
机构信息
Department of Clinical Nutrition, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; College of Health Science and Technology, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai, China; Shanghai Institute of Pediatric Research, Shanghai, China.
School of Life Sciences, Westlake University, Hangzhou, China; Westlake Intelligent Biomarker Discovery Lab, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, China.
出版信息
Am J Clin Nutr. 2023 Sep;118(3):561-571. doi: 10.1016/j.ajcnut.2023.07.019. Epub 2023 Jul 29.
BACKGROUND
Longitudinally conserved microbe-microbe interactions may provide insights to understand the complex dynamic system of early-life gut microbiota among preterm infants.
OBJECTIVES
We aimed to profile the covarying network of gut microbiota among preterm infants and investigate its potential influence on host growth (2-5 y).
METHODS
We collected time-series stool samples (n = 717 from children and n = 116 from mothers) among 51 preterm and 51 full-term infants from birth up to 5 y of age and among 53 mothers. The included infants underwent time-series measurements of early-life gut microbiota (0-5 y) and growth (2-5 y) from June 2014 to April 2017. The covarying taxa that exhibited consistent covariation from day 1 to year 5 were defined as conserved features in the development of gut microbiota. Childrens' height-for-age z score (HAZ) and weight-for-age z score were calculated according to World Health Organization Child Growth Standards.
RESULTS
We observed distinct dynamic patterns of both microbial alpha and beta diversity comparing preterm infants with full-term controls during the very early stage (<3 mo). Moreover, we identified a covarying network containing 10 taxa as a conserved gut microbial feature of these preterm infants from birth to 5 y old. This covarying network was distinctive between preterm and full-term infants before 3 mo of age (P < 0.001) and tended to be similar as the infants grew up. Several covarying taxa of the network during early life (<3 mo) were associated with childhood growth (2-5 y) (eg, Clostridium_sensu_stricto_1 with HAZ, β = -0.32, q < 0.01), and the human milk feeding duration was a main modulating factor.
CONCLUSIONS
Preterm born children possess conserved and distinct covarying microbiota during very early life, which may have a profound influence on their growth later in life. This trial was registered at clinicaltrials.gov as NCT03373721.
背景
纵向保守的微生物-微生物相互作用可能为理解早产儿早期肠道微生物组的复杂动态系统提供线索。
目的
我们旨在描绘早产儿肠道微生物组的共变网络，并研究其对宿主生长（2-5 岁）的潜在影响。
方法
我们收集了 51 名早产儿和 51 名足月产婴儿以及 53 名母亲从出生到 5 岁的时间序列粪便样本（儿童 n = 717，母亲 n = 116）。纳入的婴儿在 2014 年 6 月至 2017 年 4 月期间进行了早期肠道微生物组（0-5 岁）和生长（2-5 岁）的时间序列测量。从第 1 天到第 5 年表现出一致共变的共变分类群被定义为肠道微生物组发育的保守特征。根据世界卫生组织儿童生长标准计算儿童的身高年龄 z 评分（HAZ）和体重年龄 z 评分。
结果
我们观察到早产儿和足月产对照婴儿在非常早期阶段（<3 个月）的微生物 alpha 和 beta 多样性具有明显不同的动态模式。此外，我们鉴定出一个包含 10 个分类群的共变网络，作为这些早产儿从出生到 5 岁的肠道微生物特征。这个共变网络在 3 个月前的早产儿和足月产婴儿之间是独特的（P < 0.001），并且随着婴儿的成长，这种网络的模式趋于相似。网络中早期生活（<3 个月）的几个共变分类群与儿童生长（2-5 岁）有关（例如，Clostridium_sensu_stricto_1 与 HAZ，β = -0.32，q < 0.01），人乳喂养时间是一个主要的调节因素。
结论
早产儿在生命早期具有保守和独特的共变微生物组，这可能对他们以后的生长有深远的影响。本试验在 clinicaltrials.gov 上注册为 NCT03373721。
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