Institute of Process Equipment and Environmental Engineering, School of Mechanical Engineering and Automation, Northeastern University, Shenyang 110819, China.
Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.
Bioresour Technol. 2023 Nov;387:129575. doi: 10.1016/j.biortech.2023.129575. Epub 2023 Jul 28.
Hyperthermophilic composting (HC) has been widely recognized for the advantage of high treatment efficiency for organic wastes. However, the humification process is still unclear. In this study, the humification process of HC was investigated, compared to conventional composting (CK). The results showed that the highest composting temperature, organic matter degradation rate, and humification index in HC were 92.62 °C, 23.98%, and 1.59, while those in CK were 70.23 °C, 14.49 %, and 1.04, indicating HC accelerated humification process. Moreover, the results of metagenomic and untargeted metabolomic showed that the genes and metabolisms related to carbohydrate, lipid, amino acid, fatty acid, and nucleotide were more abundant in HC. Consequently, the metabolic pathways regarding organic matter degradation and microbial reproduction were enhanced in the high temperature stage of HC, further accelerating the humification reaction in the low temperature stage. This work contributes to the comprehension of the humification mechanism in HC.
嗜热堆肥（HC）因其对有机废物处理效率高的优势而得到广泛认可。然而，其腐殖化过程仍不清楚。本研究对比了常规堆肥（CK），调查了 HC 的腐殖化过程。结果表明，HC 的最高堆肥温度、有机质降解率和腐殖化指数分别为 92.62°C、23.98%和 1.59，而 CK 分别为 70.23°C、14.49%和 1.04，表明 HC 加速了腐殖化过程。此外，宏基因组和非靶向代谢组学的结果表明，HC 中与碳水化合物、脂质、氨基酸、脂肪酸和核苷酸相关的基因和代谢物更为丰富。因此，HC 高温阶段的有机质降解和微生物繁殖相关代谢途径得到增强，进一步加速了低温阶段的腐殖化反应。本工作有助于理解 HC 中的腐殖化机制。
