Deng Junhao, Meng Fanqi, Gao Jianpeng, Zhang Kexue, Liu Zhongyang, Li Ming, Liu Xiao, Li Jiantao, Wang Yu, Zhang Licheng, Tang Peifu
Department of Orthopedics, The Chinese PLA General Hospital, Beijing, 100853, China.
School of Life Sciences, Tsinghua University, Beijing, 100084, China.
Heliyon. 2023 Jul 12;9(7):e18158. doi: 10.1016/j.heliyon.2023.e18158. eCollection 2023 Jul.
Spinal cord injury (SCI) is a devastating disorder that often results in severe sensorimotor function impairment with limited recovery of function. In recent years, rehabilitation training for spinal cord injury has gradually emerged, and some of them play an important role in the repair of spinal cord injury However, the optimal training regimen for SCI remains to be determined. In this study, we explore the effects of rotarod training (began at 7 days post-injury) on the recovery of motor function after SCI, as well as its possible repair mechanism from the aspects of function and histopathological changes, the behaviors of specific trophic factors and cytokines, and the expression profile of specific genes. Multiple functional assessments showed that rotarod training initiated at 7 days post-injury is unsuitable for promoting neuro-electrophysiological improvement and trunk stability, but impaired functional coordination and motor recovery. In addition, rotarod training has negative effects on spinal cord repair after SCI, which is manifested as an increase of lesion area, a decrease in neuronal viability, a deterioration in immuno-microenvironment and remyelination, a significant reduction in the expression of trophic factors and an increase in the expression of pro-inflammatory factors. RNA sequencing suggested that the genes associated with angiogenesis and synaptogenesis were significantly downregulated and the PI3K-AKT pathway was inhibited, which was detrimental to spinal cord repair and impeded nerve regeneration. These results indicate that immediate rotarod training after SCI is currently unsuitable for rehabilitation in mice.
脊髓损伤（SCI）是一种毁灭性疾病，常导致严重的感觉运动功能障碍，功能恢复有限。近年来，脊髓损伤的康复训练逐渐兴起，其中一些训练在脊髓损伤修复中发挥着重要作用。然而，SCI的最佳训练方案仍有待确定。在本研究中，我们探讨了转棒训练（在损伤后7天开始）对SCI后运动功能恢复的影响，以及从功能和组织病理学变化、特定营养因子和细胞因子的行为以及特定基因的表达谱方面探讨其可能的修复机制。多项功能评估表明，损伤后7天开始的转棒训练不利于促进神经电生理改善和躯干稳定性，反而损害了功能协调性和运动恢复。此外，转棒训练对SCI后的脊髓修复有负面影响，表现为损伤面积增加、神经元活力降低、免疫微环境和髓鞘再生恶化、营养因子表达显著降低以及促炎因子表达增加。RNA测序表明，与血管生成和突触形成相关的基因显著下调，PI3K-AKT通路受到抑制，这不利于脊髓修复并阻碍神经再生。这些结果表明，SCI后立即进行转棒训练目前不适用于小鼠的康复。
