Wang Huijuan, Sun Deshun, Lin Weiming, Fang Chao, Cheng Kui, Pan Zhengzhou, Wang Daping, Song Zhangfa, Long Xiaojun
Department of Colorectal Surgery, Key Laboratory of Biological Treatment of Zhejiang Province, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310016, China.
Southern University of Science and Technology Hospital, Intelligent Medical Innovation Center, Shenzhen, 518035, China.
Bioact Mater. 2023 Jun 20;28:420-431. doi: 10.1016/j.bioactmat.2023.06.005. eCollection 2023 Oct.
Full-thickness skin wounds are have continued to be reconstructive challenges in dermal and skin appendage regeneration, and skin substitutes are promising tools for addressing these reconstructive procedures. Herein, the one-step fabrication of a cell sheet integrated with a biomimetic hydrogel as a tissue engineered skin for skin wound healing generated in one step is introduced. Briefly, cell sheets with rich extracellular matrix, high cell density, and good cell connections were integrated with biomimetic hydrogel to fabricate gel + human skin fibroblasts (HSFs) sheets and gel + human umbilical vein endothelial cells (HUVECs) sheets in one step for assembly as a cell sheet-laden hydrogel (CSH). The designed biomimetic hydrogel formed with UV crosslinking and ionic crosslinking exhibited unique properties due to the photo-generated aldehyde groups, which were suitable for integrating into the cell sheet, and ionic crosslinking reduced the adhesive force toward the substrate. These properties allowed the gel + cell sheet film to be easily released from the substrate. The cells in the harvested cell sheet maintained excellent viability, proliferation, and definite migration abilities inside the hydrogel. Moreover, the CSH was implanted into a full-thickness skin defects to construct a required dermal matrix and cell microenvironment. The wound closure rate reached 60.00 ± 6.26% on the 2nd day, accelerating mature granulation and dermis formation with skin appendages after 14 days. This project can provide distinct guidance and strategies for the complete repair and regeneration of full-thickness skin defects, and provides a material with great potential for tissue regeneration in clinical applications.
全层皮肤伤口在真皮和皮肤附属器再生方面仍然是重建挑战，而皮肤替代物是解决这些重建手术的有前景的工具。在此，介绍了一种一步法制备的集成有仿生水凝胶的细胞片，作为用于皮肤伤口愈合的组织工程皮肤。简要地说，将具有丰富细胞外基质、高细胞密度和良好细胞连接的细胞片与仿生水凝胶一步集成，制备凝胶+人皮肤成纤维细胞（HSFs）片和凝胶+人脐静脉内皮细胞（HUVECs）片，用于组装成载细胞片水凝胶（CSH）。通过紫外光交联和离子交联形成的设计仿生水凝胶由于光生醛基而表现出独特性能，这些醛基适合整合到细胞片中，并且离子交联降低了对底物的粘附力。这些性能使得凝胶+细胞片薄膜能够容易地从底物上释放。收获的细胞片中的细胞在水凝胶内保持优异的活力、增殖能力和明确的迁移能力。此外，将CSH植入全层皮肤缺损处，构建所需的真皮基质和细胞微环境。第2天伤口闭合率达到60.00±6.26%，14天后加速成熟肉芽组织和带有皮肤附属器的真皮形成。该项目可为全层皮肤缺损的完全修复和再生提供独特的指导和策略，并为临床应用中的组织再生提供具有巨大潜力的材料。
