Wang Guangli, Chen Mengqing, Jiang Li, Zhang Yunfang
Anhui Province Key Laboratory of Pollution Sensitive Materials and Environmental Remediation, School of Life Sciences, Huaibei Normal University, Huaibei, China.
State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China.
Front Microbiol. 2023 Jul 13;14:1209322. doi: 10.3389/fmicb.2023.1209322. eCollection 2023.
Nitenpyram is a neonicotinoid insecticide that is commonly found in the environment. However, its biodegradation by pure cultures of bacteria has not been widely investigated and the catabolic pathway (s) for nitenpyram metabolism remain elusive. In this study, the aerobic strain DF-1, isolated from a wastewater-treatment pool contaminated with nitenpyram. The strain was designated an sp. utilizing a combination of traditional methods and molecular ones. Strain DF-1 can use nitenpyram as a sole carbon or nitrogen source for growth. In liquid medium, 100 mg·L nitenpyram was metabolized to undetectable levels within 10 days. Four metabolites were found by gas chromatography-mass spectrometry (GC-MS) analyses during nitenpyram degradation. According to the aforementioned data, a partial metabolic pathway of nitenpyram was proposed of strain DF-1. Inoculation of strain DF-1 promoted nitenpyram (10 mg·kg) degradation in either sterile or non-sterile soil. To our knowledge, this is the first characterization of nitenpyram degradation by a specific bacterium and likely to be exploited for the remediation of nitenpyram-contaminated sites.
烯啶虫胺是一种在环境中普遍存在的新烟碱类杀虫剂。然而，其在细菌纯培养物中的生物降解尚未得到广泛研究，烯啶虫胺代谢的分解代谢途径仍然不明。在本研究中，从受烯啶虫胺污染的废水处理池中分离出好氧菌株DF-1。利用传统方法和分子方法相结合的方式将该菌株鉴定为一个新种。菌株DF-1能够利用烯啶虫胺作为唯一碳源或氮源进行生长。在液体培养基中，100 mg·L的烯啶虫胺在10天内被代谢至检测不到的水平。在烯啶虫胺降解过程中，通过气相色谱-质谱联用（GC-MS）分析发现了四种代谢产物。根据上述数据，提出了菌株DF-1对烯啶虫胺的部分代谢途径。接种菌株DF-1促进了无菌或非无菌土壤中烯啶虫胺（10 mg·kg）的降解。据我们所知，这是首次对特定细菌降解烯啶虫胺进行表征，并且可能用于修复受烯啶虫胺污染的场地。
