Merdy Patricia, Delpy Floriane, Bonneau Adrien, Villain Sylvie, Iordachescu Lucian, Vollertsen Jes, Lucas Yves
Université de Toulon, Aix Marseille University, CNRS, IM2NP, 83041, Toulon, France.
Aalborg University, Department of the Built Environment, Thomas Manns Vej 23, 9220, Aalborg, Denmark.
Heliyon. 2023 Jul 17;9(8):e18387. doi: 10.1016/j.heliyon.2023.e18387. eCollection 2023 Aug.
Studies on the environmental impact of nanoplastics face challenges in plastic analysis and a scarcity of nanoplastic materials necessary for the development of analytical techniques and experiments on biota impact. Here we provide detailed procedures for obtaining nanoparticles suspended in water for the most commonly used polymers: Polypropylene (PP), Polyvinylchloride (PVC), Low- and High-Density Polyethylene (PE-LD, PE-HD), and Polystyrene (PS). We dissolved larger size material to reprecipitate nanoparticles. For all plastic types, we obtained nanoparticles with a size between 50 and 300 nm, and a mainly spherical morphology. We verified that no irreversible agglomeration or coalescence of the particles occurred after 5 days of storage. The concentrations obtained in the final carrier solution were of the order of 10 particles mL. To prevent the persistence of reagents in the final carrier solution, a filtration step was implemented at the end of the process. The method proved unsuitable for Polyethylene Terephthalate (PET).
纳米塑料对环境影响的研究在塑料分析方面面临挑战，且缺乏用于开发分析技术和生物群影响实验所需的纳米塑料材料。在此，我们提供了详细的程序，用于获取悬浮在水中的纳米颗粒，这些纳米颗粒由最常用的聚合物制成：聚丙烯（PP）、聚氯乙烯（PVC）、低密度和高密度聚乙烯（PE-LD、PE-HD）以及聚苯乙烯（PS）。我们将较大尺寸的材料溶解以重新沉淀出纳米颗粒。对于所有塑料类型，我们获得了尺寸在50至300纳米之间且主要为球形形态的纳米颗粒。我们验证了颗粒在储存5天后没有发生不可逆的团聚或聚结。最终载体溶液中获得的浓度约为每毫升10个颗粒。为防止试剂在最终载体溶液中残留，在该过程结束时实施了过滤步骤。该方法被证明不适用于聚对苯二甲酸乙二酯（PET）。
