Phase I Clinical Trial Center, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China.
Department of Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, 250117, China.
Funct Integr Genomics. 2023 Jul 31;23(3):257. doi: 10.1007/s10142-023-01183-0.
In recent years, the potassium voltage-gated channel subfamily D (KCND) channels, particularly KCND2 (also known as Kv4.2), have been suggested to play a role in a variety of cancers, but their role in breast cancer has not yet been revealed. We analyzed RNA sequencing data from The Cancer Genome Atlas database and the Genotype-Tissue Expression database to investigate the differential expression of KCND2 in breast cancer and normal breast tissue. In addition, we leveraged GO and KEGG analysis techniques to gain a better understanding of the potential functional enrichment of 500 genes related to KCND2. Our findings were validated using collected tissue samples and clinical data from hospitals showed that KCND2 is a crucial independent factor in the prognosis of breast cancer patients. The higher the expression of KCND2, the shorter the survival time of breast cancer patients. Colony formation assay confirmed that KCND2 promotes the proliferation of breast cancer cells, whereas transwell assay and wound healing assay verified that KCND2 promoted breast cancer invasion and migration. In addition, 5-Ethynyl-2'-deoxyuridine (EdU) and flow cytometry revealed that KCND2 affected the cycle changes of breast cancer cells and contributed to the G1/S phase transition of breast cancer cells. Overall, our study demonstrates that KCND2 holds a promising potential as a significant target for breast cancer diagnosis and therapy.
近年来，钾电压门控通道亚家族 D（KCND）通道，特别是 KCND2（也称为 Kv4.2），被认为在多种癌症中发挥作用，但它们在乳腺癌中的作用尚未被揭示。我们分析了来自癌症基因组图谱数据库和基因型组织表达数据库的 RNA 测序数据，以研究 KCND2 在乳腺癌和正常乳腺组织中的差异表达。此外，我们利用 GO 和 KEGG 分析技术，更好地理解与 KCND2 相关的 500 个基因的潜在功能富集。我们的发现通过从医院收集的组织样本和临床数据进行了验证，结果表明 KCND2 是乳腺癌患者预后的一个重要独立因素。KCND2 表达越高，乳腺癌患者的生存时间越短。集落形成实验证实 KCND2 促进乳腺癌细胞的增殖，而 Transwell 实验和划痕愈合实验验证了 KCND2 促进乳腺癌的侵袭和迁移。此外，5-乙炔基-2'-脱氧尿苷（EdU）和流式细胞术揭示 KCND2 影响乳腺癌细胞的周期变化，并促进乳腺癌细胞的 G1/S 期转变。总的来说，我们的研究表明 KCND2 作为乳腺癌诊断和治疗的一个有前途的重要靶点。
