Department of Physiology, Faculty of Medicine, Karabuk University, Karabuk, Turkey.
Department of Medical Biochemistry, Faculty of Medicine, Karabuk University, Karabuk, Turkey.
Tissue Cell. 2023 Oct;84:102182. doi: 10.1016/j.tice.2023.102182. Epub 2023 Jul 26.
Doxorubicin (DXR) is widely used in cancer treatment. However, it has not yet been possible to prevent the side effects of DXR. The aim of this study was to investigate the hepatoprotective effect of crocin against DXR used in cancer treatment. For this reason; forty Wistar rats (male-250-300 g) were allocated into four groups (n = 10/group): Control, Crocin, DXR and DXR+Crocin. Control and Crocin groups were administered saline and crocin (40 mg/kg, i.p) for 15 days, respectively. DXR group, cumulative dose 12 mg/kg DXR, was administered for 12 days via 48 h intervals in six injections (2 mg/kg each, i.p). DXR+Crocin group, crocin (40 mg/kg-i.p) was administered for 15 days, and DXR was given as in the DXR group. The results revealed that serum liver markers (alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) increased significantly after DXR administration but recovered after crocin therapy. In addition, lipid peroxidation (MDA), and inflammatory cytokine (TNF-α) increased after DXR application and the antioxidative defense system (GSH, SOD, CAT) significantly decreased and re-achieved by crocin treatment. Our results conclude that crocin treatment was related to ameliorated hepatocellular architecture and reduced hepatic oxidative stress and inflammation in rats with DXR-induced hepatotoxicity.
多柔比星（DXR）广泛用于癌症治疗。然而，目前还不可能预防 DXR 的副作用。本研究旨在探讨藏红花素对癌症治疗中使用的 DXR 的肝保护作用。为此；将四十只 Wistar 大鼠（雄性，250-300g）分为四组（n=10/组）：对照组、藏红花素组、DXR 组和 DXR+藏红花素组。对照组和藏红花素组分别给予生理盐水和藏红花素（40mg/kg，ip）15 天。DXR 组，累积剂量 12mg/kg DXR，通过 48 小时间隔的六次注射（每次 2mg/kg，ip）在 12 天内给予。DXR+藏红花素组，藏红花素（40mg/kg-ip）给予 15 天，DXR 给予与 DXR 组相同的剂量。结果表明，DXR 给药后血清肝标志物（丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和碱性磷酸酶（ALP））显著升高，但在藏红花素治疗后恢复正常。此外，DXR 应用后脂质过氧化（MDA）和炎性细胞因子（TNF-α）增加，抗氧化防御系统（GSH、SOD、CAT）显著降低，藏红花素治疗后恢复正常。我们的结果表明，藏红花素治疗与 DXR 诱导的肝毒性大鼠肝组织形态改善、肝氧化应激和炎症减少有关。
