Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, 510642 Guangzhou, China.
Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, 510642 Guangzhou, China.
J Hazard Mater. 2023 Oct 5;459:132161. doi: 10.1016/j.jhazmat.2023.132161. Epub 2023 Jul 27.
To comprehensively elucidate the ecology of the bacterial community and antibiotic resistance genes (ARGs) in urbanised coastal ecosystems, this study investigated the variations of bacterial community and five common types of ARGs, the impacting factors and assembly of bacterial community, as well as their co-occurrence relationships in two ecosystems of the Pearl River Estuary (PRE). The bacterial community composition and structure of the nearshore ecosystem (NSE) and the eight mouths of the PRE (EPR) markedly differed, with 38 phyla shared between these two ecosystems. The abundances of 10 ARGs and bacterial community diversity were significantly higher in the EPR than NSE. Moreover, 67.82% and 27.82% of the variation in the bacterial community was explained by spatial (44.42%/8.63%) and environmental (23.40%/19.19%) variables in the NSE and EPR, respectively. Significant distance-decay patterns were observed, and distinct stochastic processes (undominated processes or dispersal limitation) dominated bacterial community assembly in the NSE and EPR. Furthermore, co-occurrence patterns showed significant positive correlations between 48/182 ASVs belonging to 6/15 bacterial phyla and 8/11 ARGs in the NSE/EPR, with six common dominant hosts. These results clarify the drivers and mechanism shaping the bacterial community, providing further proof for potential ARG bacterial hosts in urbanised estuarine ecosystems.
为了全面阐明城市化沿海生态系统中的细菌群落和抗生素抗性基因（ARGs）的生态学特性，本研究调查了珠江口两个生态系统（近岸生态系统和 8 个河口）中细菌群落的变化、五种常见类型的 ARGs、细菌群落的影响因素和组装，以及它们的共现关系。近岸生态系统（NSE）和珠江口（EPR）的细菌群落组成和结构明显不同，这两个生态系统共有 38 个门。EPR 中 10 种 ARGs 的丰度和细菌群落多样性明显高于 NSE。此外，NSE 和 EPR 中细菌群落的变异分别有 67.82%和 27.82%可由空间（44.42%/8.63%）和环境（23.40%/19.19%）变量解释。在 NSE 和 EPR 中均观察到显著的距离衰减模式，且明显的随机过程（非主导过程或扩散限制）主导了细菌群落的组装。此外，共现模式显示 NSE/EPR 中 6/15 个细菌门的 48/182 个 ASVs 与 8/11 个 ARGs 之间存在显著的正相关关系，有六个共同的优势宿主。这些结果阐明了塑造细菌群落的驱动因素和机制，为城市化河口生态系统中潜在的 ARG 细菌宿主提供了进一步的证据。
